2CCQ,2HPL


Conserved Protein Domain Family
PUB_PNGase

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cd10459: PUB_PNGase 
Click on image for an interactive view with Cn3D
PNGase/UBA or UBX (PUB) domain of the P97 adaptor protein Peptide:N-glycanase (PNGase)
This PUB (PNGase/UBA or UBX) domain is found in the p97 adaptor protein PNGase (Peptide:N-glycanase). The PUB domain functions as a p97 (also known as valosin-containing protein or VCP) adaptor by interacting with the D1 and/or D2 ATPase domains. The type II AAA+ ATPase p97 is involved in a variety of cellular processes such as the deglycosylation of ERAD substrates, membrane fusion, transcription factor activation and cell cycle regulation through differential binding to specific adaptor proteins. Peptide:N-glycanase (PNGase), a deglycosylating enzyme that functions in proteasome-dependent degradation of misfolded glycoproteins which are translocated from the endoplasmic reticulum (ER) to the cytosol during ERAD, associates with the ubiquitin-proteasome system proteins mediated by the N-terminal PUB domain. PNGase is present in all eukaryotic organisms; however, the yeast PNGase ortholog does not contain the PUB domain. The mammalian PNGase binds a considerable number of proteins via its PUB domain; these include ERAD E3 enzyme, the autocrine motility factor receptor (AMFR or gp78), SAKS and Derlin-1.
Statistics
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PSSM-Id: 198417
Aligned: 13 rows
Threshold Bit Score: 145.494
Created: 21-Apr-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
peptide binding
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:peptide binding site [polypeptide binding site]
Evidence:
  • Comment:The C-terminal peptide of p97 fits into a hydrophobic pocket in the PUB domain
  • Structure:2HPL: Mus musculus C-terminal peptide of P97 binds to PNGase; contacts at 4.0A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         #  ##  #        ## ###  # #   #                                
2CCQ_A         7 VAELCQNtpETFLEASKLLLTYADNILRNPNDEKYRSIRIgNTAFSTRLLPVrGAVECLFEMGFEEGETHLIFPkk-aSV 85  human
2HPL_A         6 VAELCQNtpETFLEASKLLLTYADNILRNPSDEKYRSIRIgNTAFSTRLLPVrGAVECLFEMGFEEGETHLIFPkk-aSV 84  house mouse
Q5ZJM3        16 VRELCKNarDTFLEASRLLLTYADNILRNPYEEKYRSIRNgNPAFSTRLLPVrGAVECLFEMGFQEGETHMVFPke-aSI 94  chicken
Q503I8         7 VLALCENttEVFLDVSKLLITYADNILRNPNEEKYRSIRIgNPTFSTKLLPVkGAVECLFEMGFEEAETHLVFPrs-aSV 85  zebrafish
Q08B23         9 VDELCQNerQEFLDASQLLLTYADNILRSPNEEKYRSIRIgNTAFSTRLLPVrGAIECLFEMGFEEEETHLVFPkm-aSV 87  African clawed ...
XP_001625471   1 VNQLKGNsaVVFMETSELLLKFANNIMGEPENPKYRSIRLgNKIFQNKLLPVeGAVECLFAMGFEESEDRLIFPsn-cSL 79  starlet sea ane...
XP_001602886   9 LTLLQENqeDVKKEAHHILLKLCNNILKHPQDLKYRKIKIdNPVVSIKLLPAaGAIECLFEAGFVENDDEFFLPpn-aSL 87  jewel wasp
XP_002110933   7 IRLIEKNsgKVFDDASYAILRYTSNILKEPEIIKFRKIKTtNDIFKNRILPAdGAIECLLAMGFRQEDGNYILPlq-yPL 85  Trichoplax adha...
XP_002427006   9 LNILENNslEKYIESTEILIRIANNVLKEPLNPKFRSISFsSNTFTKKLLPAnGAVECLQEMGFQKDKDNLVLNhq-tSL 87  human body louse
XP_002605291   8 IRLLVSNptSVFLDCSEILLRYAENIIRNPQEPKYRTIRLeNQVVRSRLVPVpGALDCLKAMGFTQASERQFYArlesSF 87  Florida lancelet
EFN85481       9 LRCLEENeyDICDKAQRALLNICHKLLMSPDDDKCREVRFdNEIIIQKILPAvGAIECLVEVGYVEDGERLFFPhd-tSL 87  Harpegnathos sa...
EFZ13792       9 VKSLEENteDVRDEARRALLNICQNVLRSPGNYRSRELRLdDEVVIEKLLPAvGAMECLFDVGYVEDGERIFLPrn-tSL 87  red fire ant
XP_392561      9 LDLLKENdeQVRNDAENALLTVCQNILSHPNDKKFREVRLdHPLVTAKLLPAlGAIECLFDIGFIETTDCLSLPpe-aPL 87  honey bee
Feature 1                      
2CCQ_A        86 EQLQKIRDLIAIER 99  human
2HPL_A        85 EQLQKIRDLIAIER 98  house mouse
Q5ZJM3        95 EQLRKVRDLIAVER 108 chicken
Q503I8        86 EKLRQVRETIAAER 99  zebrafish
Q08B23        88 EKLRNVRDHIAIER 101 African clawed frog
XP_001625471  80 EALHTLRDTLEAMR 93  starlet sea anemone
XP_001602886  88 TGVRDLYSLLNSSQ 101 jewel wasp
XP_002110933  86 HDLTDIHQSLINER 99  Trichoplax adhaerens
XP_002427006  88 DSIKYIHDELIKRR 101 human body louse
XP_002605291  88 QHVLMYEDPVLQAQ 101 Florida lancelet
EFN85481      88 SRIQHLSQMLSAKL 101 Harpegnathos saltator
EFZ13792      88 LKLQSLSRLLSTTI 101 red fire ant
XP_392561     88 SKVQTLQKLLNKNS 101 honey bee

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