1V34,1V33,1G71,1ZT2


Conserved Protein Domain Family
AE_Prim_S

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cd04860: AE_Prim_S 
Click on image for an interactive view with Cn3D
AE_Prim_S: primase domain similar to that found in the small subunit of archaeal and eukaryotic (A/E) DNA primases. Primases are DNA-dependent RNA polymerases which synthesis the short RNA primers required for DNA replication. In addition to its catalytic role in replication, DNA primase may play a role in coupling replication to DNA damage repair and in checkpoint control during S phase. In eukaryotes, this small catalytically active primase subunit (p50) and a larger primase subunit (p60), referred to jointly as the core primase, associate with the B subunit and the DNA polymerase alpha subunit in a complex, called Pol alpha-pri. The function of the larger primase subunit is unclear. Included in this group are Pfu41 and Pfu46, these two proteins comprise the primase complex of the archaea Pyrococcus furiosus; Pfu41 and Pfu46 have sequence identity to the eukaryotic p50 and p60 primase proteins respectively. Pfu41 preferentially uses dNTPs as substrate. Pfu46 regulates the primase activity of Pfu41.
Statistics
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PSSM-Id: 240130
Aligned: 57 rows
Threshold Bit Score: 193.984
Created: 7-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:nucleotide binding site [chemical binding site]
Evidence:
  • Structure:1V34_A, Pyrococcus horikoshii DNA primase small subunit bound with uridine 5-prime-triphosphate (UTP)

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
1V34_A        32 KNFYTNEWkvkdi-------pdfiVKTLELREFGFDhs----geGPSDRKNQYTDIRDLEDYIRATAPY-----AVYSSV 95  Pyrococcus hori...
NP_523972     41 PHLPFYRWlsyg---------sseDAIFSNREISFTlq-----dDIYIRYLCFDTQAELEKEICSRNPIkidigPVMHSK 106 fruit fly
XP_001121983  20 PFHDYYRWlsy-----------gnVNTFNNREFSFTlq-----gDVYIRFQSFKNVKELELQIRRLLPVkidvgAVYNYS 83  honey bee
NP_958856     24 PFSQYFRWlsyg---------gvsKNYFQNREFSFTlk-----dDIYVRYQSFSSQNELEKEMQKMVPYkidigAVYSHR 89  zebrafish
BAD97111      22 PYSQYYRWlnyg---------gviKNYFQHREFSFTlk-----dDIYIRYQSFNNQSDLEKEMQKMNPYkidigAVYSHR 87  human
XP_791278     32 PFGPYYRWlnyg---------gapKSYFLNREFSFTle-----nDIYIRYQSFSDQNEMEKEIQKRCPYkidigAVFTHK 97  purple urchin
AAH49395      24 PFYQYFRWlnyg---------gvvKNYFQHREFSFTlk-----dDVYVRYQSFNNQSELEKEMQKMCPYkidigAVYSHR 89  African clawed ...
AAM89973      29 PSKGMYDWltyyqpemkdtegkpkSDYFYRREFSFTle-----dGIYVRYNCFKNEEEFKQTLIEKSPEki-diGAVFNM 102 Moneuplotes cra...
CAL56541      61 PAEALFKWlshgnde---rhakadKTYAGKREFCFVldkengdgEIFCRYQCFEDAGRFAREVRSKNPAriefgPVMNAK 137 Ostreococcus tauri
XP_971054     24 PHTALHKWlcygn-------psgdPNVSNRREISFTlv-----gDIYLRYQSFENAEDFAQALHKRFPVkidigAIYKQR 91  red flour beetle
Feature 1                              # # #                                              #  ## #
1V34_A        96 AlyekPQem---eGWLGTELVFDIDAKDLplrrcehepgtVCPICLNDAKEIVRDTVIILREELGFNdIHIIYSG-RGYH 171 Pyrococcus hori...
NP_523972    107 PknhrSIpg--glTPVQRELVFDIDMTDYdevrtccsgagVCLKCWKFMVLAARVLDVALREDFGFEhIIWIFSGrRGIH 184 fruit fly
XP_001121983  84 PkeqkMRgt--nfHPVEKELVFDIDMTDYdeirtccsgadICASCWKFMALACKILDTALRSDFGYKhILWVFSGrRGIH 161 honey bee
NP_958856     90 PsqhnTVksg-tfQALEKELVFDIDMTDYddvrrccsaadICSKCWTLMTIAIRILDRALREDFGFHhLLWVYSGrRGVH 168 zebrafish
BAD97111      88 PnqhnTVklg-afQAQEKELVFDIDMTDYddvrrccssadICPKCWTLMTMAIRIIDRALKEDFGFKhRLWVYSGrRGVH 166 human
XP_791278     98 PkdhrTLkaa-afQAQEKELVFDIDMTDYddvrsccqgadICSKCWPLMTIAIKIVDSALYEDFGFEhRLWVYSGrRGVH 176 purple urchin
AAH49395      90 PslhnTVksg-tfQAQEKELVFDIDMTDYddvrrccssadICPKCWTLMTIAVRILDRALAEDFGFKhRLWVYSGrRGVH 168 African clawed ...
AAM89973     103 PpknhSSvesrafIPQEKELVFDIDMTDYddvrtcceganVCLKCWRFMSIATKIIDKAMREDFGYKhLLWVFSGrRGVH 182 Moneuplotes cra...
CAL56541     138 PahrhSVk----lHSVERELVFDIDMTDYddvrtcckdaqICGKCWPLMTIAIKILDAGLRRDFGFKhLLWVYSGrRGVH 213 Ostreococcus tauri
XP_971054     92 LessgSYss--skTPVEREIVFDIDMTDYddvrtcctgagVCPKCWKFMVIACKILDGALREDFGFEnILWVFSGrRGIH 169 red flour beetle
Feature 1                                                                                        
1V34_A       172 IRVLDEWALKLDSKSRERILSFVSASEiedveefrklllnkrgwfvlNHGYPRAFRlrfgyfilriklphlinagirksi 251 Pyrococcus hori...
NP_523972    185 CWVCDYQARHLDGRGRYAVAEYLNIITyasfaggns---prcsmgdrPHHSLKRALkivepmfeeivledqnlfgtpkgv 261 fruit fly
XP_001121983 162 CWVCDPCARTLTARERGAVAEYLQIIDggeymkkk-----vnlpgdkIHHSIKRALetieavfvpf-------------- 222 honey bee
NP_958856    169 CWVCDDAARKLSVAARSAVAEYLSLVKggeetlr------kvvlsdpIHPFISRSLsvvehyfpqyaivdqdilgnkesv 242 zebrafish
BAD97111     167 CWVCDESVRKLSSAVRSGIVEYLSLVKggqdvkk------kvhlsekIHPFIRKSIniikkyfeeyalvnqdilenkesw 240 human
XP_791278    177 CWVCDEAARKLSQNARSAIAEYLSIVKggenqvk------kvslpdqLHPSVERAKqvivsyfedyalnkqdilgdkesw 250 purple urchin
AAH49395     169 CWVCDDSARKLSQAERSAVAEYLSVVKggeetik------kvqlpetIHPFIGKSLkmveryfekyalvdqdilenkqcw 242 African clawed ...
AAM89973     183 CWVCDPSTRKLTNEARSAIANYLTVHVgnemgst------takidfvAHPAISRAKdiikeeftdimmrgqnilskeeqr 256 Moneuplotes cra...
CAL56541     214 CWVSDERARKLSDEARAAVAEYFAVVKgqgksrr-------vysinpMHPSVKRAYddvlkkywietylpeqrilennak 286 Ostreococcus tauri
XP_971054    170 CWVADKKARILDDNQRSAIAEYLTLLRgglvvr--------kvkffnMHAATERALkvideyfeksilveqdilgtyerl 241 red flour beetle
Feature 1                                                                                #       
1V34_A       252 aksilkskeeiy------------------------eefvrkailaafpqgvgieslaklfalstrfsksyFDGRVTVDL 307 Pyrococcus hori...
NP_523972    262 tkllnmvhddaargelesymqknledgahsrlvwesfikyansmrtsttsawsrklknivaeiqlgllyprLDINVTRGF 341 fruit fly
XP_001121983 223 -------------------------------------------------------------cieeqnilgtDDGVENKGM 241 honey bee
NP_958856    243 dkvlallpedikkdlieyy---------qnhkepmkrwdrlrqlvdhkkssskkggqyydkeimlqycyprLDVNVSKGV 313 zebrafish
BAD97111     241 dkilalvpetihdelqqsfq-------kshnslqrwehlkkvasryqnnikndkygpwleweimlqycfprLDINVSKGI 313 human
XP_791278    251 kkvlalvpdalrghlendmt--------kgsnslerwkllrervsrevsvgrakrcgqlvaeiafqycyprLDVNVSKGV 322 purple urchin
AAH49395     243 dkvialvpevaresllref----------skarssverwdklsscleatgkdfrrysnipkeimlqfcyprLDVNVSKGL 312 African clawed ...
AAM89973     257 kkliemlpeeevrnhlktnwdry-tdyedqensvklwnifteiagdkggklrksyrtdyvtsamitflyprIDANVSKGI 335 Moneuplotes cra...
CAL56541     287 ldqvldlledqelkdelvdefng-skmssverwrvieervmqarqpkqgvkrnytldfalekiifwhiyprVDIEVSKHM 365 Ostreococcus tauri
XP_971054    242 daflgnidegfsplfrdamt-------kvqtsverwkafvntfqtlqreqkipkhyrymveemkfyyayprLDINVTKSL 314 red flour beetle
Feature 1         ## #     #                      
1V34_A       308 KRILRLPSTLHSKVGLIAKYVgtnerdvMRFNP 340 Pyrococcus horikoshii
NP_523972    342 NHLLKAPFCIHPATGKVCVPFsvs--avAKFDP 372 fruit fly
XP_001121983 242 NHLLKSPFCIHPKTGKVCIPFtan--tvDKFHP 272 honey bee
NP_958856    314 NHLLKSPFSVHPKTGRISVPIdlk--elDTFDP 344 zebrafish
BAD97111     314 NHLLKSPFSVHPKTGRISVPIdlq--kvDQFDP 344 human
XP_791278    323 NHLLKSPFCVHPKTGRVCVAMdta--elDRFDP 353 purple urchin
AAH49395     313 NHLLKSPFSVHPKTGRISVPIdck--klDQFDP 343 African clawed frog
AAM89973     336 NHLLKSPFCVHPKTGKICVPFdps--kvDEFDP 366 Moneuplotes crassus
CAL56541     366 NHLLKGPFCVHPKTGRVCVPMdpa--kaEFFDP 396 Ostreococcus tauri
XP_971054    315 NHLLKAPFCIHPKSGKVSVPFkpk--fiEHFDP 345 red flour beetle

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