1PRX,1XCC,2CV4


Conserved Protein Domain Family
PRX_1cys
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cd03016: PRX_1cys 
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Peroxiredoxin (PRX) family, 1-cys PRX subfamily; composed of PRXs containing only one conserved cysteine, which serves as the peroxidatic cysteine. They are homodimeric thiol-specific antioxidant (TSA) proteins that confer a protective role in cells by reducing and detoxifying hydrogen peroxide, peroxynitrite, and organic hydroperoxides. As with all other PRXs, a cysteine sulfenic acid intermediate is formed upon reaction of 1-cys PRX with its substrates. Having no resolving cysteine, the oxidized enzyme is resolved by an external small-molecule or protein reductant such as thioredoxin or glutaredoxin. Similar to typical 2-cys PRX, 1-cys PRX forms a functional dimeric unit with a B-type interface, as well as a decameric structure which is stabilized in the reduced form of the enzyme. Other oligomeric forms, tetramers and hexamers, have also been reported. Mammalian 1-cys PRX is localized cellularly in the cytosol and is expressed at high levels in brain, eye, testes and lung. The seed-specific plant 1-cys PRXs protect tissues from reactive oxygen species during desiccation and are also called rehydrins.
Links
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Statistics
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PSSM-Id: 239314
Aligned: 55 rows
Threshold Bit Score: 293.677
Created: 4-Feb-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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catalytic triaddimer interfacedecamer
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:
  • Comment:Deprotonation of the catalytic cysteine thiol is facilitated by H-bonding with the threonine or serine hydroxyl group, and the positive charge of the arginine sidechain.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                              #  #                                     
1PRX_A        7 LGDVAPNFEANTTvGRIRFH-DFLGd-SWGILFSHPRDFTPVXTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSK 84  human
O67024        9 LGEPAPAFEAQTTfGPVKFPdDFKG--QWVVLFSHPADFTPVCTTEFVAFAKNYEEFKKRNVQLIGLSVDSNFSHIAWVM 86  Aquifex aeolicus
NP_623740    17 IGAPAPDFKANSTfGPIKLS-DYRG--KWVVLFSHPGDFTPVCTTEFIAFTQVYTSFVERNVQLIGLSVDSNPSHLAWVE 93  Thermoanaerobact...
Q974S8        4 LYSKFPDVQVLTTkGPIDFYkDIFGkgKWLFLFAHPADFTPVCTTEFVAFSQKYEEFKKLGVELVGLSVDSIYSHIQWLM 83  Sulfolobus tokodaii
YP_182278    11 IGDIAPQFEALTThGVLKLE-DFKG--QWLIIFSHPADFTPVCTTEFIAFSEIYPELQKRGVELLGLSVDSNSSHIAWVR 87  Dehalococcoides ...
ZP_00280897  16 LYEPAPDFEARSTmGMVSSA-DYRG--KWLLFFSHPADFTPVCTSELIAFARIAPRFKELGCELLALSIDGLYSHLAWLR 92  Burkholderia fun...
NP_662627     4 LGDDFPELKVQTThGPMNIPgDLKG--SWFVLFSHPADFTPVCTTEFVAFQQRVEAFEKIGCKLIGMSVDQVFSHIKWVE 81  Chlorobium tepid...
NP_970628    11 LGDDFPQLAVSTThGPMKLPcDLKG--SWFVLFSHPADFTPVCTTEFVAFQKLMPEFEKLGVKLIGLSIDQIQSHLKWIE 88  Treponema dentic...
Q9HJL3        1 MGQKAPDFTVNTSkGPVTLS-SYRG--KWVLLFSHPGDFTPVCTTEFIAFTERYKDFQALGVELLGLSIDSVYSHIAWIR 77  Thermoplasma aci...
ZP_00639651  11 LNRPAPAFNAKTThGMRSLT-DYKG--KWLVLFSHPADFTPVCTTEFIGFAQRAEEFAHINTELLGLSIDSVHSHIAWVR 87  Shewanella frigi...

Feature 1                                                      #                                
1PRX_A       85 DINAYnseepteKLPFPIIDDRNrELAILLGMLdpaekdekgmPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVV 164 human
O67024       87 NIKEKfg----iEIPFPIIADHNmEVAKKYGMIhpa----qstTFTVRALFVIDDKGILRAMIYYPLTTGRNIREVIRLV 158 Aquifex aeolicus
NP_623740    94 NIYKTtg----vEIPFPIIEDKDmRIAKLYGMIspa----etsTSAVRAVFIIDDKQILRLILYYPLEIGRNIQEIIRII 165 Thermoanaerobact...
Q974S8       84 DIEQRyg----vKVPFPVIADPDkKLARMLDALde------asGQTIRIVVLASPDGIIRFVAQYPMEFGRNIDELLRIT 153 Sulfolobus tokodaii
YP_182278    88 NVEEKtg----iKIPFPIIADLNkEVSLAYGMIhpg----qskTETVRCVFILDPDQKIRLIMYYPMNVGRNMKEILRVI 159 Dehalococcoides ...
ZP_00280897  93 SIQERfn----mDIPFPVVEDPSmAVAKAYGMLppk----avsSSTVRGMFLIDPEGIIKAISWYPISTGRSVEEAFRLF 164 Burkholderia fun...
NP_662627    82 WIKENld----vDITFPIVAAND-RIANKLGMLhpg-----kgTNTVRAVFVGDPNGKVRLVLYYPQEIGRNMDEILRAV 151 Chlorobium tepid...
NP_970628    89 WIKEKlg----vEITFPVIAAND-SIANQIGLLhpg-----kgTNTVRAVFIVDPNGKVRLVLYYPQEIGRNMEEIVRAV 158 Treponema dentic...
Q9HJL3       78 DIKEHfg----iEIPFPIIADIDkEVARQYNLIde------ksGATVRGVFIIDPNQIVRWMIYYPAETGRNIDEIIRVI 147 Thermoplasma aci...
ZP_00639651  88 NIEENfg----vDITFPIISDLSmEVAKAYGMIqpg----asdTSAVRATFIIDPEGMLRAMVYYPMSNGRSIDEFVRLV 159 Shewanella frigi...

Feature 1                                                                             
1PRX_A      165 ISLQLTAEKRVATPVDWKd----------gDSVMVLPTipeeeak--klfpkgVFTKElpsgkkYLRYTP 222 human
O67024      159 DALQTADREGVATPADWVpepqtwefteenTKVIVPPPttyedav--krlqegYECADw-----YICKKK 221 Aquifex aeolicus
NP_623740   166 DALQTVDKYKVLAPANWYp----------gMPVIVPPPktypelkqrlknvegYTCTDw-----YLCYKK 220 Thermoanaerobacter tengcon...
Q974S8      154 KAAIVNYKAKVVLPANWQp----------gQDVIVPPPaifdea---emriklPNAKAw-----YLLFKK 205 Sulfolobus tokodaii
YP_182278   160 DALQTADENKVALPANWHp----------gDKVVVPPPstqemae--ermgqgYECIDw-----YLCKKN 212 Dehalococcoides ethenogene...
ZP_00280897 165 QAVRMTWQENLYAPADWQp----------gSPCVVPPPktieda---skrlseGDVLDw-----YYQTRQ 216 Burkholderia fungorum LB400
NP_662627   152 KVLQISDSNKVAMPADWPnnl------likDHVIIPPAnnvedak--krkeqqYDCYDw-----WFCHKP 208 Chlorobium tepidum TLS
NP_970628   159 KALQTSDKNKVALPADWPnng------likDRAIIPPPpteaea---kkrlkeYDGYDf-----WFCHKS 214 Treponema denticola ATCC 3...
Q9HJL3      148 KALQFNWERKLATPANWQpg---------qEGIEGAPStvdas-----lkrdgEPGVKt----wYLKFVK 199 Thermoplasma acidophilum
ZP_00639651 160 KALQTSDANGVATPENWQp----------gDDVIVPPPattadak--arlsqgYDYVDw-----YFSKKS 212 Shewanella frigidimarina N...

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