1G7R,3WBI,4UPY,1G7S


Conserved Protein Domain Family
IF2_aeIF5B_IV

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cd16266: IF2_aeIF5B_IV 
Click on image for an interactive view with Cn3D
Domain IV of prokaryotic Initiation Factor 2 and archaeal and eukaryotic Initiation Factor 5
This family represents the domain IV of prokaryotic Initiation Factor 2 (IF2) and its archaeal and eukaryotic homologs IF5B. IF2, the largest initiation factor is an essential GTP binding protein. In E. coli three natural forms of IF2 exist in the cell, IF2alpha, IF2beta1, and IF2beta2. Disruption of the eIF5B gene (FUN12) in yeast causes a severe slow-growth phenotype, associated with a defect in translation. eIF5B has a function analogous to prokaryotic IF2 in mediating the joining of the 60S ribosomal subunit. The eIF5B consists of three N-terminal domains (I, II, II) connected by a long helix to domain IV. Domain I is a G domain, domain II and IV are beta-barrels and domain III has a novel alpha-beta-alpha sandwich fold. The G domain and the beta-barrel domain II display a similar structure and arrangement to the homologous domains in EF1A, eEF1A and aeIF2gamma.
Statistics
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PSSM-Id: 293911
Aligned: 34 rows
Threshold Bit Score: 101.854
Created: 3-Aug-2015
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
tRNA binding16S rRNA
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:tRNA binding site [nucleic acid binding site]
Evidence:
  • Structure:4UPY; Oryctolagus cuniculus 80S initiation complex, contacts 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                   ### ######                                   ###                  ####
1G7R_A        464 ASIRLipKLVFRQSKPAIGGVEVLtGVIRQGYPLXndd---------getVGTVeSXQDKg-ENLKSAS---RGQKVAXA 530  Methanothermo...
3WBI_A        470 CVLQT--LQIINKRGPMIIGVDVLeGTLRVGTPICavktdpttkerqtliLGKViSLEINh-QPVQEVKkgqTAAGVAVR 546  Saccharomyces...
4UPY_B        499 CKMKIlpQYIFNSRDPIVMGVTVEaGQVKQGTPMCvpsk-------nfvdIGIVtSIEVNh-KQVDVAK---KGQEVCVK 567  rabbit
EAL46920      762 CIAEIlpQYVFNDRNPIICGVKVLeGTMKVGCPITlpsr-------nnmdLGRVtGIELNn-KPVEVGK---TGEEVCIK 830  Entamoeba his...
NP_001033585 1092 CKMKIlpQFIFNSRDPIVMGVVVEaGQVKQGTPMCvpsk-------nfvdIGIVtSIEINh-KPVEVAK---KGQEVCVK 1160 chicken
AAF47774     1016 CKLRIlpQFVFNSRDPIVMGVMVEnGIVKVGTPICvpsk-------efvdIGIVtSIESNh-KQIEFAR---KGQEICVK 1084 fruit fly
CAD98509      708 CILKIipQYIFNARDPIICGVYVEeGILKPGTPLCipek-------dnlmIGRVtSVEFNk-KPVNEGK---KGQEVAVK 776  Cryptosporidi...
CAF95765     1068 VKLRIlpQFIFNSRDPIVMGVCIDaGVLRQGTPLCvpsk-------gfvdIGVItSIELNh-KAIDCAK---KGQEVCVK 1136 Tetraodon nig...
XP_641984     921 CILEV--TNVFRNSNPILVGVRVKeGTLRIGTPICvpes-------ncadVGKViGIKLNe-KDVTLAK---KDDVVSVA 987  Dictyostelium...
A9A813        468 AMIKIlpNCIFRQKEPAVCGVEVLyGTLKIGSPLMsed---------gkkLGYVkEIRDNqqENIKEAK---VGMQVPVS 535  Methanococcus...
Feature 1         #            #            
1G7R_A        531 IKDav------ygKTIHEGDTLYVDI 550  Methanothermobacter thermautotrophicus
3WBI_A        547 LEDpsgqq-piwgRHVDENDTLYSLV 571  Saccharomyces cerevisiae S288c
4UPY_B        568 IEPipgespkmfgRHFEATDILVSKI 593  rabbit
EAL46920      831 IEPfspssvftykKHFDSKDKLYSKI 856  Entamoeba histolytica HM-1:IMSS
NP_001033585 1161 IEPipgespkmygRHFEATDILVSKI 1186 chicken
AAF47774     1085 IDPipgespkmfgRHFEADDMLISKI 1110 fruit fly
CAD98509      777 IQPfasdtnitygRHFDHNDKLVSRI 802  Cryptosporidium parvum
CAF95765     1137 IEPipgeapkmygRHFEATDVIVSKI 1162 Tetraodon nigroviridis
XP_641984     988 IDDnntk--ttiyRHFDDKKQWMSKI 1011 Dictyostelium discoideum AX4
A9A813        536 IDGniv-----lgRNAKENDILYVEV 556  Methanococcus maripaludis

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