Conserved Protein Domain Family
RJL
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cd04119: RJL 
Rab GTPase family J-like (RabJ-like)
RJLs are found in many protists and as chimeras with C-terminal DNAJ domains in deuterostome metazoa. They are not found in plants, fungi, and protostome metazoa, suggesting a horizontal gene transfer between protists and deuterostome metazoa. RJLs lack any known membrane targeting signal and contain a degenerate phosphate/magnesium-binding 3 (PM3) motif, suggesting an impaired ability to hydrolyze GTP. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization.
Links
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Statistics
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PSSM-Id: 133319
Aligned: 13 rows
Threshold Bit Score: 239.565
Created: 27-Aug-2005
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:Based on sequence similarity with other Rab isoforms.
  • Comment:The active conformation of Rab is stabilized by interations between the gamma phosphate of GTP and two critically conserved residues, Thr in switch I and Gly in switch II
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1             #######         #      ##                                               
AAH88700   17 IKVISMGNAEVGKSCIIKRYCEKRFVp-KYQATIGIDYGVTKVQik-----------------------------drEIK 66  African clawed frog
AAP45327   24 IKIISIGSSGVGKSCVIKRYCEGRFVs-KYIPTIGIDYGVKRVSlrapahm-------------------spappnfFVR 83  Trypanosoma cruzi
DAA01321   17 IKVISMGNAEVGKSCIIKRYCEKRFVp-KYLATIGIDYGVTKVQvr-----------------------------drEIK 66  chicken
DAA01381   17 VKVISLGNAEVGKSCIIKRYCEKRFVp-KYLATIGIDYGVTKVQvr-----------------------------drEIK 66  zebrafish
AAH34049   17 IKVISMGNAEVGKSCIIKRYCEKRFVs-KYLATIGIDYGVTKVHvr-----------------------------drEIK 66  human
DAA02062   39 VKIISMGESGVGKSCVIKRYCEEKFVs-KYISTIGIDYGVKPVMvn-----------------------------gaEVR 88  potato pink rot agent
DAA01320   13 VKIISMGNAEVGKSCIIKRYCERRFVs-KYLPTIGIDYGVTKVSvk-----------------------------dkEVK 62  Ciona intestinalis
EAN80241   22 IKIVSLGSVGVGKSCLIKQYCEGRFVs-KYIPTIGIDYGVKRVDvrvpahl--------------------apsgkiSTR 80  Trypanosoma brucei
DAA01328   39 IKVLTIGNTKVGKSCIVKKYCEKNRFvsNYNATIGVDYGVKSVSrtmdd------------------------gsnlNIK 94  Thalassiosira pseu...
AAL87242   15 LKVTTAGMSQVGKSCLIKYYCEGRFVk-KYLPTIGVDYGVRALDle-----------------------------gcTVR 64  Giardia intestinalis
CAI39283    8 IKVISVGDTQSGKSCLIKRFCEGRFIn-KYVTTIGVDYGVKKMMiq-----------------------------nkKVA 57  Paramecium tetraur...
AAS66271  111 HMHTVKIELKHFKSCIIKRYCEKRFVs-KYLATIGIDYGVTKNTnvdldly-------------------kdhligkLND 170 Norway rat
CAJ02739   72 IKLLVIGDVGVGKSCLIKRYCEGRFVa-KYIPTIGIDFGVKKVEvskaavlqqrssepsppaataqsgggassaippAVR 150 Leishmania major

Feature 1            #                                                                        
AAH88700   67 VNIFDMAGh--PFFY-EVRNEFYKDTQGVILVYDVGQKESFESLDAWLAEMKQElgpqin-------------------- 123 African clawed frog
AAP45327   84 VNFWDMAGr--DEFL-EIRNEFYYAVEGVLLFYDVTDASSFAALDEWLKEMQTYvnaprstgge---------------- 144 Trypanosoma cruzi
DAA01321   67 VNIFDMAGh--PFFY-EVRNEFYKDTQGVILVYDVGQKESFDALDAWLAEMKQElgphg--------------------- 122 chicken
DAA01381   67 VNIFDMAGh--PFFY-EVRNEFYKDSQGVILVYDVGLRESFDALDSWLTEMKQEmgsqa--------------------- 122 zebrafish
AAH34049   67 VNIFDMAGh--PFFY-EVRNEFYKDTQGVILVYDVGQKDSFDALDAWLAEMKQElgphg--------------------- 122 human
DAA02062   89 VNFWDLSGl--PEYL-EVRNEFYKDTQGGILMFDVSSRRSFEALDSWLREAAKFga------------------------ 141 potato pink rot agent
DAA01320   63 VNIFDMAGh--PYFY-EVRNEFYRDAQAALLVFDVCDRNSYETLESWLLEMKQEmgrnn--------------------- 118 Ciona intestinalis
EAN80241   81 VNFWDMSGc--EEYL-EIRNEFYRATEGVLLVYDVTDAESFFALNQWVKEMEAHvntkgndty----------------- 140 Trypanosoma brucei
DAA01328   95 VDFFDLSGd--PNYL-EVRNEFYTGVDAVLVIFDVTDKQSFESLSRWMKEAKQYgva----------------------- 148 Thalassiosira pseu...
AAL87242   65 INFFDLSGl--DEYQ-DIRKEFYSDTQGLLLIFDMSNRDSFTALEKYYGEFIKSstgag--------------------- 120 Giardia intestinalis
CAI39283   58 INFFDLSG---DEDYkEIRNPFFADAQGVVLVCDLTDRQSFQNLPKWEKMMTDNgld----------------------- 111 Paramecium tetraur...
AAS66271  171 VSILLNTVvicQSRL-AVRNEFYKDTQGVILVYDVGQKDSFDALDSWLAEMKQElgphg--------------------- 228 Norway rat
CAJ02739  151 VNFWDGSGd--GDYR-EILNEFYEAAQGVLLMYDARNAQSFAALQSWWEELTVYcqgmpaasggggggsgsgggggsnpa 227 Leishmania major

Feature 1                                                  ## #                               
AAH88700  124 --------------------------------idnldnIVFAVCANKIDStk--------hRCVDESEGRLWSESKg-fL 162 African clawed frog
AAP45327  145 ----------------------------tlgvnpvkkpAMVVVCANKVDNevdg----gkkRVVSEADGRRWAEAHg-yK 191 Trypanosoma cruzi
DAA01321  123 ----------------------------------nmdnIVFVVCANKIDCtk--------hRSVDESEGRLWAESRg-fL 159 chicken
DAA01381  123 ----------------------------------nmenIIFVVCANKVDLtk--------rRVVDESEGRLWAESRg-fH 159 zebrafish
AAH34049  123 ----------------------------------nmenIIFVVCANKIDCtk--------hRCVDESEGRLWAESKg-fL 159 human
DAA02062  142 ------------------------------------gkFPCILCGNKVDRp----------RVVSEDEAKAYALSKg-fE 174 potato pink rot agent
DAA01320  119 ----------------------------------kmdnIVFFVCANKIDKg---------kRMVETLEAQLWAEEKg-fK 154 Ciona intestinalis
EAN80241  141 -----------------------------varvdasvpCKVVVCANKIDEvsegg--grkkRAVSSETGRQWAKEHd-yK 188 Trypanosoma brucei
DAA01328  149 -----------------------------------sesTTLIVVGNKIDQl---------gRSVSEQQGISFASENn-aI 183 Thalassiosira pseu...
AAL87242  121 ---------------------------------agagsVSIYVIGTKADLp----------AAVSVDEATTYASRLk-gV 156 Giardia intestinalis
CAI39283  112 -----------------------------------lkqAAVYVVGNKSDL-----------KNKEVDSGDILAYAKk--K 143 Paramecium tetraur...
AAS66271  229 ----------------------------------nmenIVFVVCANKIDCsk--------hRCIDESEGRLWAESRg-fL 265 Norway rat
CAJ02739  228 lagnaaaagaggkrasasnivtsgtaagkavgrtdgraPIVVLCANKVDDtavpgaaaprpRAVSEEQGRAWAREHgcaA 307 Leishmania major

Feature 1         ###                     
AAH88700  163 YFETSAQs---GEGINEMFQAFYSSIVD 187 African clawed frog
AAP45327  192 YFETSACt---GLHVTEMLETLFNDVVA 216 Trypanosoma cruzi
DAA01321  160 YFETSAQt---GEGINEMFQTFYSAIID 184 chicken
DAA01381  160 YFETSAQs---GEGINEMFQSFFSSITD 184 zebrafish
AAH34049  160 YFETSAQt---GEGINEMFQTFYISIVD 184 human
DAA02062  175 YFETSACt---GVSITEAFHSLFRTVVK 199 potato pink rot agent
DAA01320  155 YFETSAAt---GDGITELFQALFSTLVE 179 Ciona intestinalis
EAN80241  189 YFETSACt---GAGVEEALETLFKDVVA 213 Trypanosoma brucei
DAA01328  184 YFETSAQt---GASVDDLFDYLLRETCK 208 Thalassiosira pseudonana
AAL87242  157 FFKVSSCt---GDGVAPALNTIFKQLFS 181 Giardia intestinalis
CAI39283  144 GFEGMICsaagGENVNEVNSNQNCSYLR 171 Paramecium tetraurelia
AAS66271  266 YFETSAQt---GEGINEMFQTFYMSIVD 290 Norway rat
CAJ02739  308 YYETSASt---GQNVKEVIEDLVVWMVA 332 Leishmania major

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