Conserved Protein Domain Family
HOOK

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pfam05622: HOOK 
HOOK protein coiled-coil region
This family consists of several HOOK1, 2 and 3 proteins from different eukaryotic organizms. The different members of the human gene family are HOOK1, HOOK2 and HOOK3. Different domains have been identified in the three human HOOK proteins, and it was demonstrated that the highly conserved NH2-domain mediates attachment to microtubules, whereas this central coiled-coil motif mediates homodimerization and the more divergent C-terminal domains are involved in binding to specific organelles (organelle-binding domains). It has been demonstrated that endogenous HOOK3 binds to Golgi membranes, whereas both HOOK1 and HOOK2 are localized to discrete but unidentified cellular structures. In mice the Hook1 gene is predominantly expressed in the testis. Hook1 function is necessary for the correct positioning of microtubular structures within the haploid germ cell. Disruption of Hook1 function in mice causes abnormal sperm head shape and fragile attachment of the flagellum to the sperm head. This entry includes the central coiled-coiled domain and the divergent C-terminal domain.
Statistics
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PSSM-Id: 461694
Aligned: 18 rows
Threshold Bit Score: 526.95
Created: 22-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
B6MFW3       176 ELNEVRAAKEEIAQRCHELDMQVQQLMEENTLMKVEKDELSDKVNQVD--------GYEDTSTP--AGRRYIQLTHQVEQ 245 Florida lancelet
NP_001006542 182 DLQEAIAEKEELAQRCQELDLQVAALQDEKNSLVSENEILNDRLEQLDd-------SLDDPNTV--VAKKYFHAQLQLEQ 252
XP_003439936 180 DLAELQTEKEALTQRCQELDLQVAVLQEERNSLLAENDVLTDRANQLD--------TFDDPSTP--SGRKHSQLQQQLET 249
Q5TZ80       180 DLTEVMAEKEELAQRCQELDMQVTVLQEERNSLLAENDLLTDRSSQLE--------TFDDPSTP--SGRKHSQLQFQLEQ 249 zebrafish
XP_001145260 184 ELNEALSAKEEIAQRCHELDMQVAALQEEKSSLLAENQVLMERLNQSD--------SIEDPNSP--AGRRHLQLQTQLEQ 253
XP_395724    183 ELQVTVDMKEQLAQKCHELDQQLSLLQEEKAALMTENKKLQERLDEFE--------NP-ESCSI----FRHSGLRKQVEA 249
EFN89605     186 ELQTAVDMKDQLAQRCHELDQQLSLLQEEKAALIVENKKLQERLDEFE--------NPEESGSI----LKYSGLRKQAEA 253
EFN60607     183 ELQVAIDMKDQLAQKCHELDQQLSLLQEEKAALIAENKKFQERLDEFE--------NPEESGSI----MKYSGLRKQVDA 250
B4I5P7       181 DSKAVQEDRDALAQKCFETEKKMLLLIDEKTNLQQELHKLQQEFARLEqhstvigdDGVSLGPVqtGSVRYNELRRQLDL 260 Drosophila sech...
Q7PWT9       175 DVKTLQEDRDTLAQKCHETNKKMLGLIEEKAALQQEIVKLQAIVGRYEnpnl-igdDGTSLGPIqlGSSRYNDLRKLVDS 253 African malaria...
B6MFW3       246 LQEETYRLETGRDEYRLKCEEMEKEILDLAGKNEELMALAAETQLLKDEMDILRQSAEKTSKYEQTIETYKKKLEDLADL 325 Florida lancelet
NP_001006542 253 LQEENFRLEAAKDDYRVHCEDLEKQLIELQHRNNELTSLAEESRALKDENDILRAAADKASKLESTVEVYRKKLQDLNDF 332
XP_003439936 250 LQEENFRLEAAKDDYRIHCEELEKQLIEVQHRNDDLTSLAEESRALKDELDVLRNCSDRVVMLEASVETYKRKLENLGDL 329
Q5TZ80       250 LQEENFRLEAAKDDYRIHCEELEKQLVELQHRNDELTSLAEESRSLKDELDILRSCSDRAVKLEASVETYRKKLEDLSDL 329 zebrafish
XP_001145260 254 LQEETFRLEAAKDDYRIRCEELEKEISELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDL 333
XP_395724    250 LKDELFKVETSRDDYRLKIELLEKEVLELQSRQEDLQKAADEANNLKDEIDALRETADKVAKYEQTIESYKKKMEDLSDL 329
EFN89605     254 LKDELFKVETSRDDYRLKVELLEKEVLELQLRQEDLQKAADEANHLKDEVDALRETVDKVAKYELTIESYKKKMEDMGDL 333
EFN60607     251 LKDELFKMETSRDDYRLKAELLEKEVLELQSRQEDLQKAADEANHLKDEIDALRETADKVAKYEIMIESYKKKMEDLSDL 330
B4I5P7       261 LKEELLQSEGAREDLKIKAQQQDTDLLHMQMRIEELMKSSAEVTTLKDEVDVLRESNDKLKICEAQLDTYKKKLEDYNDL 340 Drosophila sech...
Q7PWT9       254 LKDELLQAETARDDLKMKSMIQEKEIGELQVKIDELHAATAEIAQLKDEIDILKEANEKLKICETQLQTYKKKLEDYNDL 333 African malaria...
B6MFW3       326 RRTVEQLQEET--YRLETGLSHCELRKANTLRSQLDMYKKQVQELHGKVSEETKRADKAEFELKRSTEKLDTVQKEKQRI 403 Florida lancelet
NP_001006542 333 RRQVKSLQETNmmYMHNTVSLEDELREANAARAQLETYKRQVQELHNKLSEESKRADKLAFEMKRLEEKHEALVKEKERL 412
XP_003439936 330 KRQMKLLEENNmtYMQNTVSLEEELRKANAARAQLETYKRQVQELHRKLSEETRRADNLAFEMKKLEEKHETATKEKERI 409
Q5TZ80       330 RRQMKALEEKNmtYMHNTVSLEEELRKANAARAQLETYKRQVQELHKKLSDESRRADNLVFEMKKLQEKYDALVKERERI 409 zebrafish
XP_001145260 334 RRQVKLLEEKNtmYMQNTVSLEEELRKANAARSQLETYKRQVVELQNRLSEESKKADKLDFEYKRLKEKVDSLQKEKDRL 413
XP_395724    330 RRQVKILEDKNleYLQSKIDYEEESKRTTMLRNHLEVCKQQLAEAHHKLDEQTNKCDKLEFEGKKMEAQLSTLQRERDRL 409
EFN89605     334 KRQMKILEDKNleYLQVKLDYEEESKRTTMLRNHLEICKQQLTETQHKLDEMSGKYDRLVCEDKKKEARMNTLQRERDRL 413
EFN60607     331 KRQLKISEDKNveYLQAKLDYEEEVKRTMMLRNHLEACKQQLTEVHHRLDEQSNKYDRLEFEGKKMEAKLGALQRERDRL 410
B4I5P7       341 KKQVKILEERSadYVQQNAQFEEDAKRYANTKGQVELFKKEIQDLHAKLDAESSKNVKLEFDNKNLEGKNLALQRAKDSL 420 Drosophila sech...
Q7PWT9       334 KKQIKLQEERSadYLKQNLEYEEEAKKYAGLKGQVELYKKKIQDLHGMLDEEMGKTVRAEFEYNQLQGQLAVVQREKETL 413 African malaria...
B6MFW3       404 VNERDTLKETNEELHC-MQLQQGKamLYSTGslAGIGSNVespvGSPIPEVVPPEIKEKLIRLQHENKMLKLKAEGSDDE 482 Florida lancelet
NP_001006542 413 VIQCDALKETNEELRY-SQMQQDH--LSRTD--ASRIKSH----DNLAAELLPVEYREMFIQLQHENKMLLLQQEGSENE 483
XP_003439936 410 IIERDSLKELNEELRC-TQAQQHQ--LSQAG--MLPSSSPs--hDNLAAELMPIEYREKFIRLQHENKMLRVQQEEYERE 482
Q5TZ80       410 SIERDTLRETNEELRC-TQAQQDQ--LLLAGkyQTGSPSH----DNLAAEMLPIEYREKFIRLQHENKMLRLQQEESEKQ 482 zebrafish
XP_001145260 414 RTERDSLKETIEELRC-VQAQEGQ--LTTQGlmPLGSQESs---DSLAAEIVTPEIREKLIRLQHENKMLKLNQEGSDNE 487
XP_395724    410 IVERDALKETNEELKC-TQLQAAE---------NITKPSTd---tVTNTEMIPLEIKEELLCLKHENKMLKLKQMGN-ED 475
EFN89605     414 VIERDALRETNEELKC-AQLQAAE---------NMAKPSTvdsnSVAGTEMIPLEVKEKLLCLQLENKMLKARQKGN-EE 482
EFN60607     411 VIERDALKETNEELKCnTQLQTAE---------NMAKPSAds-sGVAGTEMVPLEIKEKLLRLQLENKMLKMKQTGN-ED 479
B4I5P7       421 LKERDNLREAVDELKC-GQLSSNTal----------------tgTTVSRELQPSATVEKLQRLEAENKALREGQGGQ--- 480 Drosophila sech...
Q7PWT9       414 LSERDTLREALDELKC-GQAVGAD-----------GVGHGgpggNTMSKELHSNDVCERIERLERENKALREGQGGQ--- 478 African malaria...
B6MFW3       483 RLAVSQAMLDDAQARTNELETENRLANQRILELQGQLEDMQTEQEEVGSPAKDqdSVALRKKLEEHMEKLKDADSQLQKK 562 Florida lancelet
NP_001006542 484 RIMELQKQLEQKQWTVNELGTEKRLNKERIGELQQQIEDLQKTLQEQGSKTEG--SSNLKQKLAAHMEKLSEVHDELQKK 561
XP_003439936 483 KIASLQAQLEEAHKTRSELDTENRLSRERVSELQQQVEDLQKALQSQASKPDD---SNLKRKLDAHMVQLNEAQDEIMKK 559
Q5TZ80       483 RITELQQQLEEARRGRSQLDTDNRLNRERISELQQQVEDLQKALQTQGAKPDD---SHLKRKLDAHMVQLNEAQDEIMKK 559 zebrafish
XP_001145260 488 KIALLQSLLDDANLRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAED--SVLLKKKLEEHLEKLHEANNELQKK 565
XP_395724    476 KLPTVQALLEDSEERLNTLRGKNRKANQRIIELENRLEEALGNQSSGDNKTDN---INLGQKITQLQDEVRKIQSEKERL 552
EFN89605     483 KLPSVQTLLDESEERLNTLREQNRKANQKIMEMEAKLEEVTMQSSNADGKSDS---GGLGQKAVQLQDELRKAQAEKERL 559
EFN60607     480 KLPSVQALLDDSEERLNTLRGQNRKANQRIMELETKLEEVMGTQTSDDNKND----AGLNQKMSQLQDELRKMQAERERL 555
B4I5P7       481 --TALAQLLDDANKRCENLREQLKTANERILSLSHA------------SQSDD--PILKESEFGKQIKQLMELNEQ---K 541 Drosophila sech...
Q7PWT9       479 --TALSQLLDDANQRNEKLREQLKSANQKILLLSQH-------------HAED---GTTKSELEMQMKQTLELGEQRSSA 540 African malaria...
B6MFW3       563 KEYIDNLEPKVSSSA-EKIQQLQEMLNKKDDDMKAMEERYKRYLEKAKSVIRTLDPKQNQSStPEVQALKNQLTEKERLI 641 Florida lancelet
NP_001006542 562 EAALAELQPDVSQNP-QKIGELEAALRKKDEDMKAMEERYKMYLEKARNVIKTLDPKLNPAS-AEIMLLRKQITERDKKI 639
XP_003439936 560 KELLEDLQPDNTQTS-LKVDELMAALKKKDDDMRAMEERYKMYLEKARNVIRALDPKLNPAT-VEIQALRNQLADRDKQI 637
Q5TZ80       560 KELLEDLQPDATQTS-VKMDELMAALKKKDEDMRAMEDRYKMYLEKARDVIRALDPKLNPAS-AEIQSLKVQLSDKDKKI 637 zebrafish
XP_001145260 566 RAIIEDLEPRFNNSS-LKIEELQEALRKKEEEMKQMEERYKKYLEKAKSVIRTLDPKQNQGAaPEIQALKNQLQERDRLF 644
XP_395724    553 ILQVEERENALQTQK-QKVFALQEKLTRREYDNAALEERYKKYVEKAKSVIKSLDPKQNNSSpNEVAVLRNQILEQRKIM 631
EFN89605     560 TLQLEEREATLQVQK-QKTATLQEKLTRKEYEDAAREEHYKKNVEKAKSVIKSLESSK--HSpTEL-VLRNQLLEQRKAI 635
EFN60607     556 TLQLEERESALQAQK-QKAFAIQEKLTRKEYENAALEERYKKYIEKAKSVIKSLDPKQNNSSpSEVVVLRNQLLEQRKMI 634
B4I5P7       542 TLQLEE-AVTQSTSLqCKVTQLETNLSAREQEILVYDAKYRKCVEKAKEVIKSIDPRI-------ASALDASVLE--KSA 611 Drosophila sech...
Q7PWT9       541 QQQLDETTQHQLSNLhTKIANLEAALVVKDQELQAADVRYKKCVEKAKEVIKTLDAHAi----SEALLM--------DKV 608 African malaria...
B6MFW3       642 DHLERD-HEKAKLt------------REQEEKLIVSAWYNMGAQLHRKAVEGRLANGG-Pm--qGGQSFLARQRQATSR- 704 Florida lancelet
NP_001006542 640 EALEAE-YKLAKL-------------RDYEENLIVTAWYNKSLTLQKLGMEARLLGSGgAcrdgPGRSFLAQQRHVTNT- 704
XP_003439936 638 LSLERQ-CEQAKL-------------REYEEKLIVTAWYNKSLNFQKLAIESRLGGHTaSml-sPGQSFLTQQRQVSNAp 702
Q5TZ80       638 IALERE-CEQAKL-------------REYEEKLIVTAWYNKSLNFQKMAIESRLSGRAnSlv-pPGQSFLAQQRQVTNA- 701 zebrafish
XP_001145260 645 HSLEKE-YEKTKSq------------REMEEKYIVSAWYNMGMTLHKKAAEDRLASTG------SGQSFLARQRQATSS- 704
XP_395724    632 EDMERS-LKESKLi------------KEMEEKLMISAFYRLGLTCHREAIDQRLAALSs----gQGQSFLARQRQPSA-- 692
EFN89605     636 EEMERT-VKESRLl------------REMEEKLMVTAFYKLGLSAQRDAMDQRLATLSs----gQGHSFLARQRQPSA-- 696
EFN60607     635 EDMERS-MKESRLl------------REMEEKLMVTAFHRLGLNCHREVIDQRLAALSs----gQGQSFLARQRQPSA-- 695
B4I5P7       612 DLVEAEpKPKMSV---------------MEEQLMTSAFYRLGVNAQRDAIDSKLAILMg-----SGQTFLARQRQSAP-- 669 Drosophila sech...
Q7PWT9       609 DSASGTgVDATLTtvgnggtaarapmGQQEEQLIATAFYRLGMVCHREAVDARL--LSg-----PGQSFLARQRQPAA-- 679 African malaria...
B6MFW3       705 RTTVS 709 Florida lancelet
NP_001006542 705 RRNLP 709
XP_003439936 703 RRALS 707
Q5TZ80       702 RRTMS 706 zebrafish
XP_001145260 705 RRSYP 709
XP_395724    693 RRHPP 697
EFN89605     697 RRHSP 701
EFN60607     696 RRHPP 700
B4I5P7       670 RKSLS 674 Drosophila sechellia
Q7PWT9       680 RKPLN 684 African malaria mosquito
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