3V0T,3UYI,3V0U,3V0S


Conserved Protein Domain Family
AKR_AKR13D1

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cd19145: AKR_AKR13D1 
Click on image for an interactive view with Cn3D
AKR13D family of aldo-keto reductase (AKR)
Rauvolfia serpentina PR is a founding member of aldo-keto reductase family 13 member D1 (AKR13D1). It catalyzes the NADPH-dependent reduction of the aldehyde perakine to yield the alcohol raucaffrinoline in the biosynthetic pathway of ajmaline in Rauvolfia, a key step in indole alkaloid biosynthesis. This family also includes Arabidopsis thaliana aldo-keto reductases, ALKR1-6.
Statistics
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PSSM-Id: 381371
Aligned: 17 rows
Threshold Bit Score: 488.481
Created: 19-Apr-2018
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitecatalytic
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3V0T: Rauvolfia serpentina perakine reductase in complex with 2'-monophosphoadenosine-5'-diphosphate, contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                        #                                                            
3V0T_A      2 PRVKLGTQGLEVSKLGFGCMGLsgdy-ndalPEEQGIAVIKEAFNCGi-TFFDTSDIYGengsNEELLGKALKqlpREKI 79  serpentwood
EFJ14664    4 PKVKLGSQGLEVSKQGLGCMGMshkvygplkPEAEMIDLIRKAVDSGv-TFLDTSDVYGph-tNEVLVGKAIQg-iRDKV 80  Selaginella moelle...
ABK24562    8 GRLQLGCEGFEVSVQGLGCMGMsygy-gppkPDEEMISLIRHAVISKgvTFLDTSEVYGpf-tNEVLVGKAIKg-iRDKV 84  Sitka spruce
O22707      8 RRIKLGSQGLEVSAQGLGCMGLtghy-gaskPETEAIALIHHAIHSGv-TFLDTSDMYGpe-tNEILLGKALKdgvREKV 84  thale cress
Q93ZN2      8 RRMKLGSQGLEVSAQGLGCMGLsafy-gapkPENEAIALIHHAIHSGv-TLLDTSDIYGpe-tNEVLLGKALKdgvREKV 84  thale cress
Q9C5B9      8 RRIKLGSQGLEVSAQGLGCMGLsifd-gttkVETDLIALIHHAINSGi-TLLDTSDIYGpe-tNELLLGQALKdgmREKV 84  thale cress
Q9ASZ9      8 RRIKLGSQGLEVSAQGLGCMGLsafy-gtpkPETEAIALIHHAIHSGv-TFLDTSDIYGpe-tNELLLSKALKdgvREKV 84  thale cress
Q84M96      8 RRMKLGSQGLEVSAQGLGCMALsary-gapkPETDAIALLHHAINSGv-TFFDTSDMYGpe-tNELLLGKALKdgvKEKV 84  thale cress
PWA80606    4 PTRKLGSQGLQVSAIGLGCLSMsqt----dkPQQEMIQLLHTAIDSGv-TFLDTSDVYGsn-aNELLIGQALKggrRDKV 77  sweet wormwood
PKU75838    8 GRIKLGSQGLEVSAQGLGCMGMsafy-gppkPEPDMIDLVHHAVRSGv-TFLDTSDIYGpf-tNETLIGKALRegwREKV 84  Dendrobium catenatum
Feature 1                                                                                     
3V0T_A     80 QVGTKFGIheigf---sgvkaKGTPDYVRSCCEASLKRLDVDYIDLFYIHRIDTTVPIEITMGELKKLVEEGKIKYVGLS 156 serpentwood
EFJ14664   81 QLATKFGVtipdsgklsgmaiRGDPAYVRAACEASLKRLQVDYIDLYYQHRVDHSVPIEITVGAMKELVEEGKVKYLGLS 160 Selaginella moelle...
ABK24562   85 QIATKFGCrfveg----kpqlRGDPAYVRAACEESLKRLDVDCIDLYYQHRVDTKVPIEVTIGEMKKLVEEGKIKYIGLS 160 Sitka spruce
O22707     85 ELATKFGIsyaeg----nreiKGDPAYVRAACEASLKRLDVTCIDLYYQHRIDTRVPIEITMGELKKLIEEGKIKYIGLS 160 thale cress
Q93ZN2     85 ELATKFGIsyaeg----krevRGDPEYVRAACEASLKRLDIACIDLYYQHRVDTRVPIEITMGELKKLVEEGKIKYIGLS 160 thale cress
Q9C5B9     85 ELATKFGLllkdq----klgyRGDPAYVRAACEASLRRLGVSCIDLYYQHRIDTTVPIEVTIGELKKLVEEGKIKYIGLS 160 thale cress
Q9ASZ9     85 ELATKYGIryaeg----kvefKGDPAYVRAACEASLMRVDVACIDLYYQHRIDTRVPIEITIGELKKLVEEGKIKYIGLS 160 thale cress
Q84M96     85 ELATKFGFfiveg---eisevRGDPEYVRAACEASLKRLDIACIDLYYQHRIDTRVPIEITMRELKKLVEEGKIKYIGLS 161 thale cress
PWA80606   78 QIATKFGIksispe--tgltvTGDPVYVKEACNGSLKRLGVDCIDLYYIHRIDTTIPIEITMGALKELVEEGKIKYVGLS 155 sweet wormwood
PKU75838   85 ELATKFGIrdggn---girevRGEPAYVRAACEGSLKRLGVDCIDLYYQHRIDTRVPIEVTMGELKKLVEEGKIKYIGLS 161 Dendrobium catenatum
Feature 1                                                     ####  #                         
3V0T_A    157 EASPDTIRRAHAVHPVTALQIEYSLWTRDIEDEIVPLCRQLGIGIVPYSPIGRGLFWGKAIKEslpens----------- 225 serpentwood
EFJ14664  161 EASAEDIRRAHAIHPITAVQLEWSLWTRDIEEEVVPTCRELGIGIVPYSPLGRGFFSGKTVAEeigdgd----------- 229 Selaginella moelle...
ABK24562  161 EASASTIRRAHAIHPITAVQLEWSLWSRDAEEEIIPTCRELGIGIVPYSPLGRGFLSSGGKLLesltds----------- 229 Sitka spruce
O22707    161 EASASTIRRAHTVHPITAVQLEWSLWTRDVEEEIVPTCRELGIGIVSYSPLGRGFFASGPKLVenldnn----------- 229 thale cress
Q93ZN2    161 EASASTIRRAHAVHPITAVQIEWSLWTRDVEEEIIPTCRELGIGIVAYSPLGRGFFASGPKLVenlekd----------- 229 thale cress
Q9C5B9    161 EACASTIRRAHAVHPLTAVQLEWSLWSRDVEEDIIPTCRELGIGIVAYSPLGLGFFAAGPKFIesmdng----------- 229 thale cress
Q9ASZ9    161 EASASTIRRAHAVHPITALQIEWSLWSRDVEEDIIPTCRELGIGIVAYSPLGRGFFASGPKLVenldnn----------- 229 thale cress
Q84M96    162 EASASTIRRAHAVHPITAVQIEWSLWSRDAEEDIIPICRELGIGIVAYSPLGRGFLAAGPKLAenlend----------- 230 thale cress
PWA80606  156 EASPETIRRAHAVHPITAVQLEWSLWTRDVEEQVIPTCRELGIGIVPFSPLGKGFFSSVPVLTedlavt----------- 224 sweet wormwood
PKU75838  162 EASASTIRRAHAVHPITAIQLEWSLWTRDSEKEIIPTCRELGIGIVAYSPLGRGFLSYGPKMVeglseqdarkdlrlsrf 241 Dendrobium catenatum
Feature 1                                                                                     
3V0T_A    226 -------------------------------------------------------------vltshPRFVGENLEKNKQI 244 serpentwood
EFJ14664  230 -------------------------------------------------------------frktiPKFQGENLERNKVV 248 Selaginella moelle...
ABK24562  230 ------------------------------------------------------------dyrktvPRFEAKNLEKNNVA 249 Sitka spruce
O22707    230 ------------------------------------------------------------dfrkalPRFQQENLDHNKIL 249 thale cress
Q93ZN2    230 ------------------------------------------------------------dfrkalPRFQEENLDHNKIV 249 thale cress
Q9C5B9    230 ------------------------------------------------------------dyrkglPRFQQENLDHNKIL 249 thale cress
Q9ASZ9    230 ------------------------------------------------------------dvrktlPRFQQENLDHNKIL 249 thale cress
Q84M96    231 ------------------------------------------------------------dfrktlPRFQQENVDHNKIL 250 thale cress
PWA80606  225 ------------------------------------------------------------dvrrqfPRFQGENLEHNKAL 244 sweet wormwood
PKU75838  242 rkfgfrgftksylnqiwdatkqpvriltlqftkiqdndseletaslcdksnklvkdpdslivlqkmPRFQSGNVEQNAGI 321 Dendrobium catenatum
Feature 1                    #                 #####  #  ##                
3V0T_A    245 YYRIEALSQKHGCTPVQLALAWVLHQGEDVVPIPGTTKIKNLHNNVGALKVKLTKEDLKEI 305 serpentwood
EFJ14664  249 YEKLCKIAAKKACTPGQLALAWVQHQGDDVVPIPGTTKLQNFEENRASLGVFLSKEDLEGI 309 Selaginella moellendorffii
ABK24562  250 FERISDIASKKGCSPGQLALSWVHHQGNDVSPIPGTTKVKNLEENIGALSVKLTHEEMKEI 310 Sitka spruce
O22707    250 YEKVSAMSEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIRALSVKLTPEEMSEL 310 thale cress
Q93ZN2    250 YEKVCAISEKKGCTPGQLALAWVHHQGDDVCPIPGTTKIENLKQNIGALSVKLTPEEMTEL 310 thale cress
Q9C5B9    250 YEKVNAMAEKKSCTPAQLALAWVHHQGNDVCPIPGTSKIKNLNQNIGALSVKLSIEEMAEL 310 thale cress
Q9ASZ9    250 FEKVSAMSEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIGALSVKLTPEEMSEL 310 thale cress
Q84M96    251 FEKVSAMAEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIRALSVKLTPEEISEL 311 thale cress
PWA80606  245 LEKVHQMAKNKDCTTSQLALAWVLYQGEDVCPIPGTTKIDNFKQNIGAVSVKPSHEDMSEL 305 sweet wormwood
PKU75838  322 FERVNSMASRKGCTPSQLALAWVHHQGNDVCPIPGTTKIENFNQNIGALSVKLSPEELAEL 382 Dendrobium catenatum

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