Old yellow enzyme (OYE)-like FMN binding domain. OYE was the first flavin-dependent enzyme identified, however its true physiological role remains elusive to this day. Each monomer of OYE contains FMN as a non-covalently bound cofactor, uses NADPH as a reducing agent with oxygens, quinones, and alpha,beta-unsaturated aldehydes and ketones, and can act as electron acceptors in the catalytic reaction. Members of OYE family include trimethylamine dehydrogenase, 2,4-dienoyl-CoA reductase, enoate reductase, pentaerythriol tetranitrate reductase, xenobiotic reductase, and morphinone reductase.
Comment:Note that in 1Z48 an arginine finger (R336) at the COOH terminus of one monomer extends into the active site of the adjacent monomer and is involved in substrate recognition
Comment:Substrate binding site based on 3 substrates: sulfate, pHBA, and pNP
Jiyao W et al.(2023). "The conserved domain database in 2023.",