5LQ4,3EO7


Conserved Protein Domain Family
McbC_SagB-like_oxidoreductase

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cd02142: McbC_SagB-like_oxidoreductase 
oxidase similar to the microcin B17 processing protein McbC
This family is the oxidase domain of NRPS (non-ribosomal peptide synthetase) and other systems that modify polypeptides by cyclizing a thioester to form a ring. These include EpoB, part of the epothilone biosynthesis pathway; TubD, part of the tubulysin biosynthesis pathway, MtsD, part of the myxothiozol biosynthesis pathway; IndC, part of the indigoidine biosynthesis pathway and TfxB, part of the trifitoxin processing pathway. All are FMN-dependent and oxidize the product of the cyclization of thioesters in short polypeptides.
Statistics
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PSSM-Id: 380318
Aligned: 45 rows
Threshold Bit Score: 85.1638
Created: 12-Sep-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
FMN bindinghomodimer
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:FMN binding site [chemical binding site]
Evidence:
  • Structure:5LQ4: the homodimer of ThcOx from Cyanothece sp . PCC 7435 binds two molecules of FMN, contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                        # # #                                                                       
5LQ4_A                   272 AIETRRSIREYsdq------------------------piTLAQLGEFLYRCARVkavytlpedp--------------- 312  Cy...
CAB87990                 760 DILSLLHEPLLtaisrq------------------pdaltLDELGHWLRYLGQFTsaerl-------------------- 801  Er...
CAC11516                  99 KNVGLEEVNLKnvllgrrttr----------ifqnesmklEDFSSILWNALSRVRhvrnl-------------------- 148  Th...
AAN85522                1646 DSGPGPVDFFAteererfkrqhwnrrpdepglpevplgdaRFEDELHAWRASRRDfldqpvphrsfsrll---------- 1715 St...
CAF28743                   5 KAMRDDSANLFskhiep------------------ilsnsQPLSEVILLRGSTRKfsrepitfeql-------------- 52   un...
YP_028801                116 KYMSEQLNRTHvaarste----------------iqlettDELPTIIKERRSCRQfemekyisfsefsqf---------- 169  Ba...
YP_001386444             105 QNVIELPPSKNi---------------------------rVGLSTAIKSRRSVRGyssksmslndvanifyytqgicdev 157  Cl...
NP_213629                301 IYGNPPILNLVps--------------------------yMSAEEVIRKRRSGMAydgktyilrelfi------------ 342  Aq...
P42725                   131 KSYPEPMESKRrkrgtatrl-----------vkpsrelaaAVILDGLMNRRSFKPftrkql------------------- 180  Rh...
PRJNA231221:FIU13_08745  106 EKIIRLPKPTKi---------------------------sTALSTCIIKRRSHRQfsdrqmplqdlsnilyyac--gvss 156 
Feature 1                                     ### #                                                          
5LQ4_A                   313 ------mqvgesttrPYPSGGALYELEIYPLVHqcg-------------------------------dlaAGLYHYQPl- 354  Cy...
CAB87990                 802 -----------lpkyTYASPGALYATQVFLELNgva-------------------------------gltAGHYYYQPv- 838  Er...
CAC11516                 149 ----------syedpLDYFASHGTEFDFYVIAYnin-------------------------------hlqNGIYLYDVk- 186  Th...
AAN85522                1716 -gllrettgadgtgaLYPSAGDTYSVQVYLHLTpdav-----------------------------egldAGLYYYDPs- 1764 St...
CAF28743                  53 ----snilysiagptPSDFGEKKSLIDVYFIANdvt-------------------------------niqKGAYFFNRn- 96   un...
YP_028801                170 -lsalrqvreekiyyHYASAGGLYPIDIFVYIKpkri-----------------------------egmkAGFYYYNPs- 218  Ba...
YP_001386444             158 epynlegnkkkiklrANPSAGGLYPIQLYVYMKnik-------------------------------dlkDGIYTYYPy- 205  Cl...
NP_213629                343 --haldktlprdnysPFDVEIGLPFVNLFIFVNrve-------------------------------nlpKGLYCFVRne 389  Aq...
P42725                   181 ---------sitevsEILWFGNLYARERAVIAEnrdfesprdiafdsafsalstfvvtygqidwqdgslpPGVYRYNVv- 250  Rh...
PRJNA231221:FIU13_08745  157 qasirdgasdkitlrNCASGGGLYPIHLVFYARnis-------------------------------kliDGFYEYLPy- 204 
Feature 1                                                                                                    
5LQ4_A                   355 -------------------------------sHTLHPvadwtpeveslvydawratgqqSIPQIVLIITARFGRLFWKYh 403  Cy...
CAB87990                 839 -------------------------------hHQLVRvseq---------------aavTPGSLRLHFVGKKSAIEPIYk 872  Er...
CAC11516                 187 -------------------------------dHFLSElksgdfreem--ihnlwwhqapKTANFTILFVTDFEQYQWRYr 233  Th...
AAN85522                1765 -------------------------------rHSLRLlrsgvlpdrg--ahfyynrpvfDRSRFGIYLFGQRHGIEPLYa 1811 St...
CAF28743                  97 -------------------------------dNSIDLlkanirrdvs--gylcleqslfSDASAVFYIMSNIKLILEKLg 143  un...
YP_028801                219 -------------------------------kNSLVIvnnidqviks--dhelinqdlfTQSAFSVYLVYNANASIPKYg 265  Ba...
YP_001386444             206 -------------------------------sHGLKPievnkellkvd-nfaefgvlnaENANLIVFYVYNFLKNSRKYg 253  Cl...
NP_213629                390 ellkdlkeitsseflwekvfedfplyllktgdFREVAgfvac------------aqdiaSDSAFSLGMISLFEPVLREEp 457  Aq...
P42725                   251 -------------------------------nHELEAiragdfkldm--aklaigqsraSSGLFTFVICGDLKSYTSRYr 297  Rh...
PRJNA231221:FIU13_08745  205 -------------------------------qHALRCyrhsseenvr--dfaeygainaENCNIIIIYVYHYMKNTRKYg 251 
Feature 1                          #  ##    #   #            ####                                          
5LQ4_A                   404 d-iaYSLILKHVGVLYQTFYLVATa--MQLAPSAIGAGN---------TTKFCQIAGLnpd-----eeASVGEFSLGA 464  Cyan...
CAB87990                 873 n-niREVLQMEMGHIIGMLDIILPdygLGVALCDAAALDptplaidldDDYLGACDVLsgprlptdddLDIYVQTAGA 949  Erwi...
CAC11516                 234 heraLRNIFIEAGRIAQRLIFEAEr--MGFGSLPTPATR---------DSSFDKMLSLkrn-----kqFVLYTITVGK 295  Ther...
AAN85522                1812 e-esLRYLTLESGYMSQLLMLGQAa--HGVGLCPIGALN---------TEQLSQWLGLde------ghVFLQAFLGGA 1871 Stre...
CAF28743                 144 n-rgYRLCQFESGILAGRIYLSAYd--QKIGASGSTFYDda------vSDFFSPHAKDk---------DVMIAIGIGV 203  uncu...
YP_028801                266 s-dgYLFACIESGIITATLNMVAEt--LNLGVCSVGHMK---------FEEIQQFLCLdn------hqVFLHGLEVGL 325  Baci...
YP_001386444             254 d-agFSYALIETGEMAQNLQLVSTa--LGYGACDIGGYE---------KQYIEEVLDIdgv-----lkHVVHMTVVGW 314  Clos...
NP_213629                458 w--fYRNLHWEAGLIGQVLYLEATa--HNLNGTGIGCFF---------DDAMHEVLGIsdk-----tfQDLYHFTFGK 517  Aqui...
P42725                   298 hersYRNLLINTSQLAQFYLTLATi--NDFNTFLTPAIH---------DEKMHLFLEAe---------DDLPLYLVTA 355  Rhiz...
PRJNA231221:FIU13_08745  252 n-qaTAYAFIESGEIAQNIQLTATa--LTYGSIDIGGYN---------KEYLQELLDLdgl-----geHVIHMTLVGT 312 

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