3A2K,1NI5


Conserved Protein Domain Family
TilS_N
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cd01992: TilS_N 
N-terminal domain of tRNA(Ile)-lysidine synthase and similar proteins
tRNA(Ile)-lysidine synthase (EC 6.3.4.19), also called tRNA(Ile)-2-lysyl-cytidine synthase or tRNA(Ile)-lysidine synthetase, catalyzes the ligation of lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. This subfamily belongs to the adenine nucleotide alpha hydrolase superfamily that also includes N-type ATP PPases and ATP sulfurylases. It forms an alpha/beta/alpha fold which binds to adenosine group. This domain has a strongly conserved motif SGGXD at the N-terminus.
Links
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Statistics
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PSSM-Id: 467496
Aligned: 184 rows
Threshold Bit Score: 102.672
Created: 12-Oct-2004
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:tRNA binding site [nucleic acid binding site]
Evidence:
  • Structure:3A2K: Geobacillus kaustophilus tRNA(Ile)-lysidine synthase binds tRNA; contacts at 4A
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                         
3A2K_A         20 AVIVGVSGGPDSLALLHVFLSLrdew----------klqVIAAHVDHMFrg----rESEEEMEFVKRFCverrilcetaq 85  
CCI41336      222 HVAISLSGGVDSMVLAYLLHKVrlss---------qyygIVAIHIDYANr-----pESAAEHTYVKEWCdrldiqfyarr 287  Albugo candida
OEU16890      488 AIIISLSGGVDSMVIVRVLAHLktscg-------ydyliVHAVHIDYANr-----pESGAEADYVRRYCeenlgtntief 555  Fragilariopsi...
XP_003062610  278 AVGISLSGGVDSMALAVILKRLsrdas-------yggfdVVAMHIDYDNr-----pESGVEADFVRGWCdrmgiecvvrk 345  Micromonas pu...
XP_014154398  249 YILLSLSGGVDSMVLAYCCLRLspll---------nnikVVCAHIDYNNr-----kESTAEANFLKQWCenlgidiviht 314  Sphaeroforma ...
XP_002780992  256 VLVLSLSGGVDSMTHLYLLSKLqqpl----------gfkLVACHIRHSNrdd--aiQELQWVTYVTARLgvplyhhhvkl 323  Perkinsus mar...
XP_002290025  528 TTIVSLSGGVDSMVIASALAYLrdteaarcnlkpdkvlrIICIHIDYANr-----pESGAEAAYVGRYChqigaqfvcrk 602  Thalassiosira...
Q3ZJ89         29 KLLIAFSGGQDSSCLLTIFYILskkw----------gfkLGAVYCNHCWtdspqssLSAFDTLQMFDIPfyfvesp---- 94   Tupiella akineta
XP_002768284   94 TVVLSLSGGVDSSSTLILLCALryhrv--------vdwkLRALYMGYSAnre-nldEARSELNLVAWLCeqcnvqlfafn 164  Perkinsus mar...
Q2EEX9         22 NVLCTFSAGQDSFFLLYCLIHIisnk----------nnkIKLQHNHHFLqss--niLSFWQCIKVASIFkiplvinlle- 88   Helicosporidi...

Feature 1                               ##  #  ##                                #   #      ##  ##
3A2K_A         86 id--------vpafqrsaglgaQEAARICRYRFFAElmek--------------hqagYVAVGHHGDDQVETILMRLVRG 143 
CCI41336      288 id--------evtrgetkrdeyEKVARDIRYSTYRDilkk--------------hgipGICFGHHRGDVQENIISNMMKG 345  Albugo candida
OEU16890      556 tcrl----igevtrgvtsrddyERIAREIRYDSYRDtvakakndmgicskeeqrntivGVMLGHHRGDLRENVLSNSHKG 631  Fragilariopsi...
XP_003062610  346 ia--------evtrgvtpreqyEAESRAIRYGFYKDvakt--------------hafpAVFVGHHEGDVQENIIANLMRG 403  Micromonas pu...
XP_014154398  315 id--------diqrgvtprdeyEKKSRDVRYNLYKKimknlykp------dgtpaavsGVLVGHHQGDVQENVISNMMKG 380  Sphaeroforma ...
XP_002780992  324 rrp------hgslktgisrmdyEKQTRDIRFSMYAKahklawaaag---lpeeertqpVVVVGHHMDDVDENRLAELGKG 394  Perkinsus mar...
XP_002290025  603 id--------evtrgvnarddyERIAREIRFGLYRQycgearrig-----gqesdigiGVMLGHHRGDLRENVISNAHKG 669  Thalassiosira...
Q3ZJ89         95 ---------------nsepmkpEQKARDWRYSSFYTiskw--------------edydFVLTGHSLSDCVETVLFNLFRG 145  Tupiella akineta
XP_002768284  165 idlprphgsegttssgvtrdeyETQTKTIRFRMYSLasgd---------------encVVVLGHHQDDVDENRLEQLSRG 229  Perkinsus mar...
Q2EEX9         89 ------------inlankdfmtENEARKWRYDSFLRnslfq-------------nekpSIFIGHTGSDLLETFFWHFLRN 143  Helicosporidi...

Feature 1         #                                                                         #     
3A2K_A        144 STskgyagipv-----------------------------------------------------krpfhggyLIRPFL-- 168 
CCI41336      346 SSllnlngmse-----------------------------------------------------tsvangvvIWRPML-- 370  Albugo candida
OEU16890      632 CGpldlsgmte-----------------------------------------------------vskndgitIYRPLL-- 656  Fragilariopsi...
XP_003062610  404 ANllavngmee-----------------------------------------------------tgvvegvaIWRPML-- 428  Micromonas pu...
XP_014154398  381 CSllevagmae-----------------------------------------------------tsvvngvtVWRPMM-- 405  Sphaeroforma ...
XP_002780992  395 NLlningmvinae------------------------------------------------gdgddeiggvcQVRPLLl- 425  Perkinsus mar...
XP_002290025  670 CGpldlsgmts-----------------------------------------------------vskndgvtLFRPLL-- 694  Thalassiosira...
Q3ZJ89        146 SGlk-------------------------------------------------------------------gICSLKEdq 158  Tupiella akineta
XP_002768284  230 HVmgdmdgmhrvr-------------------------------------------------esppgldrciVCRPLI-- 258  Perkinsus mar...
Q2EEX9        144 SIidyqlikkkllwnipyyisnfssvgyknsskvkikltnkkkkinclinhnlmlnkakksflkkdltqlvlITRPLV-- 221  Helicosporidi...

Feature 1                                         ###   #     
3A2K_A        169 AVSRAEIEAYCrqm----glsPRCDPSNEKDDYTRNRFRHHIVP 208 
CCI41336      371 QFDKSAIFDFAhr------ygIPYFKDTTPAWSTRGKLRNQLMP 408  Albugo candida
OEU16890      657 PLEKTFVLDYAhkf----gvpYFKDTILLHIGVQEEIYGDGSMN 696  Fragilariopsis cylindrus CCMP1102
XP_003062610  429 PHSKAVVLDFAhk------ygVPYFLDSTPTWSTRGKLRNQLVP 466  Micromonas pusilla CCMP1545
XP_014154398  406 PHEKKEIFDFAhk------ygIPYFKDTTPRWSTRGKLRNQLLP 443  Sphaeroforma arctica JP610
XP_002780992  426 STRKEELIACAar------hnIPYMVDSTPKWSRRGWIRGVLDL 463  Perkinsus marinus ATCC 50983
XP_002290025  695 PLEKTFIFDYAht------fgVPYFKDTTPNWSTRGKLRNRLLP 732  Thalassiosira pseudonana CCMP1335
Q3ZJ89        159 KFSNLKTNDFFfqktvcfldpFFYFSDKKGNPFFRKYRLFQSMS 202  Tupiella akineta
XP_002768284  259 HERKSTFVKVAte------ahIPYVHDSTPAWSVRGITRKALDA 296  Perkinsus marinus ATCC 50983
Q2EEX9        222 NLHRQDIFLFRknl----klpVITDKSNDNNYYYRNRIRNILFP 261  Helicosporidium sp. ex Simulium jonesi

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