3EJB,3R9C,2Z3T,3BUJ


Conserved Protein Domain Family
CYP164-like

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cd20625: CYP164-like 
Click on image for an interactive view with Cn3D
cytochrome P450 family 164 and similar cytochrome P450s
This group is composed mostly of bacterial cytochrome P450s from multiple families, including Mycobacterium smegmatis CYP164A2, Streptomyces sp. CYP245A1, Bacillus subtilis CYP107H1, Micromonospora echinospora P450 oxidase Calo2, and putative P450s such as Xylella fastidiosa CYP133 and Mycobacterium tuberculosis CYP140. CYP107H1, also called cytochrome P450(BioI), catalyzes the C-C bond cleavage of fatty acid linked to acyl carrier protein (ACP) to generate pimelic acid for biotin biosynthesis. CYP245A1, also called cytochrome P450 StaP, catalyzes the intramolecular C-C bond formation and oxidative decarboxylation of chromopyrrolic acid (CPA) to form the indolocarbazole core, a key step in staurosporine biosynthesis. CalO2 is involved in calicheamicin biosynthesis. The CYP164-like group belongs to the large cytochrome P450 (P450, CYP) superfamily of heme-containing proteins that catalyze a variety of oxidative reactions of a large number of structurally different endogenous and exogenous compounds in organisms from all major domains of life. CYPs bind their diverse ligands in a buried, hydrophobic active site, which is accessed through a substrate access channel formed by two flexible helices and their connecting loop.
Statistics
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PSSM-Id: 410718
Aligned: 88 rows
Threshold Bit Score: 316.415
Created: 29-Aug-2017
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
heme bindingchemical
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:heme binding site [chemical binding site]
Evidence:
  • Structure:3EJB: Bacillus subtilis P450(BioI) binds heme; contacts at 4A
  • Structure:2Z3T: Streptomyces sp. cytochrome P450 StaP binds heme; contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                         ##      #   
3EJB_B     26 HPIYKGSflkyPGWYVTgYEETAAILKDARFKVRTPLPEsstkyqd--------------lshvqnQMMLFQNQPDHRRL 91  Bacillus subtilis
ABE36213   27 EPVHWSPk--lSGWVVTsYELASEVLTTNGTYSAERFTAvqqhlseekrv------taaevmrwfqHWMVFRDPPDHTRL 98  Burkholderia xenov...
ABE37281   26 DPVHWSPv--lKAWVITrYDDVRRVAVSNADMSAERLAPffatvpaesqs------gfanlmtylgKWMVFRDPPEHTRL 97  Rhodopseudomonas p...
BAH47110   30 APVHWSAe--lSAWLAVdAASVQTVLTTPTVFSANRLVPvfdrlkekdqa------saadvmrwlsIWMVFQDPPNHTRL 101 Rhodococcus opacus B4
EEB78851   23 QPIWWSDge-lKGWILSrYDDVRTILKDSKTFSSQSMGEgetq--------------------amrLPLLTDDPPRHTQL 81  marine gamma prote...
CAJ88189   30 DPVHEIAa---GYWLLTrYDDVEALLRSPHSSSAFPKDPgwamsrggp---------tcpamrsvsKWLLLQDGAEHRRL 97  Streptomyces ambof...
ACU74934   33 DPVHHHPl---GFWVLSrYDDVSALLRSANSVDYRNSAPdstarlqadamieaagpatgdtvdaygMSMLDRDPPDHTRL 109 Catenulispora acid...
ABW11338   38 APAHRHPl---GFWLVSrYEDVARIQRSGHSVDERHITElpewksesrt-------lgkqnrlmhgLSMLDQDPPNHTRL 107 Frankia sp. EAN1pec
ABD53594   31 SPVHPNAd---GSVYLTrHDDCLATYRSRDMLSDKTEAFgqkfgec-------------plkvhhtTSLIFNDPPYHTVV 94  Jannaschia sp. CCS1
ACF02795   36 QPVKRLAs---GGYFLTrYDDLVAVYKNTTLFSSDKKREftpkygds------------llfehhtTSLVFNDPPSHTRV 100 Rhodopseudomonas p...
Feature 1     #      #                                                                        
3EJB_B     92 RTLASGAFtprttesyQPYIIETVHHLLDQVQgkk----kmEVISDFAFPLASFVIANIIGVpeedrEQLKEWAASLIQT 167 Bacillus subtilis
ABE36213   99 RRHMANTLnipvfdarRETVISVVNELLDRIPvgd----afDFFQAFSLWMPGIVVADLLGVerdrlLEVKQWSDDMMTF 174 Burkholderia xenov...
ABE37281   98 RRLFTKAFtsrsvmalEPNVGEIVALLFDEMEqkarstgvvDWIADFAYPLPATVMMDLLGVprddlHRVKDWSNDIALF 177 Rhodopseudomonas p...
BAH47110  102 RRHLAEVInpnmtktlREPIQEITTELLDGLPkhe----sfDFYTDFGLQLPGYVVMDLLGVprerlPEVKQWSDEMMLF 177 Rhodococcus opacus B4
EEB78851   82 RAVVNKAFtsrtlkkmEVEVAELTAELLDEMRgkt----svDISAEFTSPLPVSVIARMMGIpperkNDFKRWSDALTGT 157 marine gamma prote...
CAJ88189   98 RKLIARVFtpryidrlRPRIGEIVDGLLDSMGeg-----evDLIRDLALPMPVAVVGELLGIpvedrARCRDWTDKVGYI 172 Streptomyces ambof...
ACU74934  110 RGLVSKVFtprsvaalEPFVVDLVDAALDRMAdag----taDLIATLAFPIPFAVISRMLGIpetdhARIRVLSGLLVRT 185 Catenulispora acid...
ABW11338  108 RRLVTKAFtrravdalEGRIERIVDDALDRMAeag----rvDLVAELAFPLPFTVISELLGIpvlehGRLRELTGTMVRA 183 Frankia sp. EAN1pec
ABD53594   95 RKLIAGAFtprklkemERAIERIVSRLLDRVEdlg----elDVIADFGMMLPTEIISIMLGVpeayrAQLRGYSTAILGA 170 Jannaschia sp. CCS1
ACF02795  101 RRLIMGALtpraiagmEPDLIALVDRLLDAMAtkg----rvDLIEDFASAIPIEVIGNLLGVphderGPLRGWSLAILGA 176 Rhodopseudomonas p...
Feature 1                                                                          ##  ##  ## 
3EJB_B    168 IDftrsrk-altegNIMAVQAMAYFKELIQKRkr-----hpQQDMISMLLkgr-ekDKLTEEEAASTCILLAIAGHETTV 240 Bacillus subtilis
ABE36213  175 IGsargvpdkyeraRRGANGMGTYFLDLIAKRra-----epREDALSRLIasevegQRLSDDELVGCMMMVLNGGHETTA 249 Burkholderia xenov...
ABE37281  178 IGtsratadkylraEAGAKAMAEYFRGIIASRtv-----dpQDDIISQLVtepdkrEALTDDEVIATCILLLFAGHETTT 252 Rhodopseudomonas p...
BAH47110  178 IGsarsvenkygraRHGALAMAELFRGLIADRka-----apQDDVLTRMItseidgETLTEDELVASMMMIGNGAQETTA 252 Rhodococcus opacus B4
EEB78851  158 SEatdl-----aerMPHIMEMAGYFQSLIPQRre-----nrGEDLISKVVcaqvddQTLADQDIVGFCMLLLIAGNETTT 227 marine gamma prote...
CAJ88189  173 LDpdvtpr-rriamNKAEPEFRAYLLDLMASRrga----dpDHDLLSVLMtae-ddEQLTEEEVVANILLIFNAGHETTV 246 Streptomyces ambof...
ACU74934  186 LEpigdpa-llaeiNAARLEMQDIVRGLIAYKre-----npGEDLLTALIaaeqdgDRLSDEELIAQVVLLYVAGHETTV 259 Catenulispora acid...
ABW11338  184 LEplpdpg-lqaeiRAANDEVAAIMRAVTDWKrd-----npGDDLLTALIgaehdgDVLSAEELVAQVMLLYVAGHETTV 257 Frankia sp. EAN1pec
ABD53594  171 LDpvvsss-qmeagNRAVEEFGAVLDDLINHRranpdaaaeGEVLESLIFgeh-dgRTLTQEELVQNCIFLLNAGHETTT 248 Jannaschia sp. CCS1
ACF02795  177 LEpvigpe-afalgNAAVAEFLGYLDTLIARRtaep--gdpERDVLTRLIrgeaggEKLTAKELLHNCIFLLNAGHETTT 253 Rhodopseudomonas p...
Feature 1                                        #    ##  # #                                 
3EJB_B    241 NLISNSVLCLLQHpeQLLKLren-pdlIGTAVEECLRYESPTQMTARVASEDIDIc-gVTIRQGEQVYLLLGAANRDPsi 318 Bacillus subtilis
ABE36213  250 NLLNNSMLALAAHpqTVQHLrqh-pdeMAAAVEEFLRYDSPVLSIGRIVTEDTELg-dQEIAAGDRVFAMLVGANRDPev 327 Burkholderia xenov...
ABE37281  253 NLLGNGFYYTMNApeQWARVkdd-pslAETAVEEWLRYDGPSGALVRVVTADVEFg-gRTMLQGQRVFAFINSANRDPeq 330 Rhodopseudomonas p...
BAH47110  253 HLIGNALLALQEHpeTLAELradpakvTPGAIEEFLRYDGPVLSTARIVAEDTELg-gQHLAEGDRIFAILAAANRDPav 331 Rhodococcus opacus B4
EEB78851  228 NLLSNLLNHLADHpdTWSELrdn-pdkIDAAIEETLRYDGPVHWVNRKATRDVEFh-gQTVKAGEAVYAFMGSANRDPrh 305 marine gamma prote...
CAJ88189  247 NLIGNGMLALLRQpeALEALrad-pglMATAVDELSRFDPPVTLSSRIATAEMEFg-gKVIPPGSHVIGFLDAAGRDPer 324 Streptomyces ambof...
ACU74934  260 NLIGNGTLALLNHpeELKKLres-pqlAANGVEEMLRYDSPVQLSRRVILEPYEVg-gKVIPKGGFVVAALASANHDEsr 337 Catenulispora acid...
ABW11338  258 NLVAGGILTLLRHpdQMRRLrde-pelAGNAVEELLRYDSPVHLMRRITLEPLSVr-gTEIPPGVFVTVCLAAANRDPdf 335 Frankia sp. EAN1pec
ABD53594  249 SLVGNAVAVLLANpsEHRRLidd-ptlIGTAVEEILRVESPLQIGNRLAGEDIALpsgPTIPKGTYIHTSIAAANRDPev 327 Jannaschia sp. CCS1
ACF02795  254 NLIGNGLVTLAANpdQKQRLiae-palIKTAVEEILRYESSNQLGNRITTAEVEIg-gISMPANTSLTLCIGAANRDPaq 331 Rhodopseudomonas p...
Feature 1                        ###  ## ###   #                                           
3EJB_B    319 f-tnPDVFDITRSpn-phLSFGHGHHVCLGSSLARLEAQIAINTLLQRmPSln--laDFEWRYRPlfgFRALEELPV 391 Bacillus subtilis
ABE36213  328 f-sdPDELRTSRNpn-phMAFGKGPHFCLGTPLARLEGQIALTAILERfSSielcepVESIPWLNslvTHGPTRLPL 402 Burkholderia xenovora...
ABE37281  331 f-gdADRLDLGRSpn-phLTFGHGIHFCLGAQLARLEGQIALRALIERfPGisl-atDSAPGWRDsiiLRGMQSLPI 404 Rhodopseudomonas palu...
BAH47110  332 f-drPDELDLQRRpn-ahVAFSKGAHFCLGAPVARLEAQIALTEIVRRfPDyrisepVEEIAWSNsmvTRGPTRLPV 406 Rhodococcus opacus B4
EEB78851  306 y-edADQFSLNRGrg-dyHTFGHGIHFCIGAPLARLEARYAMEELLKRyRSish-thDANNERTHstmLRGFHHLWL 379 marine gamma proteoba...
CAJ88189  325 y-pdPDRLDLSRTep-ktLAFSAGPHFCLGAVLGRLEAATVFSKLLERyTKiel-lrEDLPVNSHf-nLHGLLELPL 397 Streptomyces ambofaci...
ACU74934  338 wgprSGDLQLDRAgarnhVAFGGGPHHCLGAALARLEGRVAVERMAVRfPAla---pAAEPVWNGrinLRGLDTLPV 411 Catenulispora acidiph...
ABW11338  336 wgpdADEVRLDRTdahrhVSFGAGIHHCVGAALARLEARVAISRFVGRfPAp----aLEDVRWNGrinVRGPASLTV 408 Frankia sp. EAN1pec
ABD53594  328 f-adPDRFDVSRKpn-phIAFITGIHVCLGASLARMEGKIAIGGFVKRfPKla---aTGEGERLGlarFRGWTSLPI 399 Jannaschia sp. CCS1
ACF02795  332 f-adPDRFDVSRSpn-rhLAFASGPHQCAGMALARLEGAIALSRFLARfPDyv---lDGPPQRGGrvrFRGYLSIPC 403 Rhodopseudomonas palu...

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