XPF-like nuclease domain of crossover junction endonuclease EME1
EME1, also called MMS4 homolog (hMMS4), interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Its typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks. The nuclease domain of EME1 is a nuclease-like domain which is involved in targeting the MUS81-EME1 heterodimer complex to DNA.
Feature 1:nucleic acid substrate binding site [nucleic acid binding site]
Evidence:
Comment:DNA-binding induced conformational changes in the linkers connecting the nuclease and nuclease-like domain of Mus81 and Eme1 facilitating the DNA substrate bending and ultimately place the incision strand at an active site of Mus81.
Structure:4P0P; Homo sapiens Mus81-Eme1 complex binds flap DNA substrates; contacts at 4A.
Jiyao W et al.(2023). "The conserved domain database in 2023.",