Conserved Protein Domain Family
CYP79

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cd20658: CYP79 
cytochrome P450 family 79
Cytochrome P450 family 79 (CYP79) enzymes catalyze the first committed step in the biosynthesis of the core structure of glucosinolates, the conversion of amino acids to the corresponding aldoximes. Glucosinolates are amino acid-derived natural plant products that function in the defense against herbivores and microorganisms. Arabidopsis thaliana contains seven family members: CYP79B2 and CYP79B3, which metabolize trytophan; CYP79F1 and CYP79F2, which metabolize chain-elongated methionine derivatives with respectively 1-6 or 5-6 additional methylene groups in the side chain; CYP79A2 that metabolizes phenylalanine; and CYP79C1 and CYP79C2, with unknown function. CYP79 belongs to the large cytochrome P450 (P450, CYP) superfamily of heme-containing proteins that catalyze a variety of oxidative reactions of a large number of structurally different endogenous and exogenous compounds in organisms from all major domains of life. CYPs bind their diverse ligands in a buried, hydrophobic active site, which is accessed through a substrate access channel formed by two flexible helices and their connecting loop.
Statistics
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PSSM-Id: 410751
Aligned: 15 rows
Threshold Bit Score: 790.796
Created: 16-Aug-2017
Updated: 25-Oct-2021
Structure
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Aligned Rows:
 
heme bindingputative
Feature 1:heme binding site [chemical binding site]
Evidence:
  • Comment:based on the structure of Salvia miltiorrhiza CYP76AH1 with bound heme (5YM3), defined at 4A contacts

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                           #                        #   #       #       
O81346        91 TEIACVKLGNTHVITVTCPKIAREILKQQDALFASRPltyaqkilsnGYKTCVITPFGDQFKKMRKVVMTELVcparhrW 170 thale cress
NP_178055     75 TDIACFRFGRVHVIVITSDVIAREVVREKDAVFADRPdsysaeyisgGYNGVVFDEYGERQMKMKKVMSSELMstkalnL 154 thale cress
NP_176122     72 TDIACYRFARFHVITVTSSKIAREVLREKDEVLADRSesyashlishGYKNISFSSYGENWKLVKKVMTTKLMspttlsK 151 thale cress
XP_006591735  77 TEIACIRLGNVHVIPVTCPSIACEFLRKHDVNFASRPltmatdimssGYVTIAIVPFGEQWKKMRRIVVNELFsplrhqW 156 soybean
Q6J541        80 TEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPkimstdiasdGFITTVLVPYGEQWKKMKRVLVNNLLspqkhqW 159 Lotus japonicus
Q6J540        80 TEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPkimstdiasdGFLTTVLVPYGEQWKKMKRVLVNNLLspqkhqW 159 Lotus japonicus
Q9M7B8        94 TDICLIRFGRTNFVPISCPVLAREILKKNDAIFSNRPktlsaksmsgGYLTTIVVPYNDQWKKMRKILTSEIIsparhkW 173 cassava
Q9FLC8        76 TDIACIRLANTHVIPVTSPRIAREILKKQDSVFATRPltmgteycsrGYLTVAVEPQGEQWKKMRRVVASHVTskksfqM 155 thale cress
Q9M7B7        93 TDICLIRFGKTNVVPISCPVIAREILKKHDAVFSNRPkilcaktmsgGYLTTIVVPYNDQWKKMRKVLTSEIIsparhkW 172 cassava
Q43135       103 TDIACVKLGGVHVVSITCPEIAREVLRKQDANFISRPltfasetfsgGYRNAVLSPYGDQWKKMRRVLTSEIIcpsrhaW 182 sorghum
Feature 1                                                      #                                 
O81346       171 LHQKRSEENDHLTAWVYnmvkns-------gsvdfRFMTRHYCGNAIKKLMFGTRTFskntapDGGPTVEDVEHMEAMFE 243 thale cress
NP_178055    155 LLKVRNLESDNLLAYVHnlynkdesktkhgavvnvRDIVCTHTHNVKMRLLFGRRHFket-tmDGSLGLMEKEHFDAIFA 233 thale cress
NP_176122    152 TLGYRNIEADNIVTYVYnlcqlgs----vrkpinvRDTILTYCHAVMMRMMFGQRHFdev-veNGGLGPKEKEHMDAIYL 226 thale cress
XP_006591735 157 LQGKRNGEADNIMFYVYnkcknvn----ngglvnvRDVAQHYCCNVTRKLIFNTRYFgkg-reDGGPGLEEVEHVNTIFT 231 soybean
Q6J541       160 LLGKRNEEADNLMFYIYnkcckdvn--dgpglvniRIAAQHYGGNVFRKLIFNSRYFgkv-meDGGPGFEEVEHINATFT 236 Lotus japonicus
Q6J540       160 LLGKRNEEADNLMFYIYnkcckdvn--dgpglvniRIAAQHYGGNVFRKLIFNTRYFgkv-meDGGPGFEEVEHINATFT 236 Lotus japonicus
Q9M7B8       174 LHDKRAEEADNLVFYIHnqfkan-------knvnlRTATRHYGGNVIRKMVFSKRYFgkg-mpDGGPGPEEIEHIDAVFT 245 cassava
Q9FLC8       156 MLQKRTEEADNLVRYINnrsvknrg--nafvvidlRLAVRQYSGNVARKMMFGIRHFgkgsedGSGPGLEEIEHVESLFT 233 thale cress
Q9M7B7       173 LHDKRAEEADQLVFYINnqyksn-------knvnvRIAARHYGGNVIRKMMFSKRYFgkg-mpDGGPGPEEIMHVDAIFT 244 cassava
Q43135       183 LHDKRTDEADNLTRYVYnlatkaat---gdvavdvRHVARHYCGNVIRRLMFNRRYFgep-qaDGGPGPMEVLHMDAVFT 258 sorghum
Feature 1                                                                                        
O81346       244 ALGFTFAFciSDYLPMLTGLDLNGhEKIMRESSAIMDKYHDPIIDERIKMWREGkrt-----------qieDFLDIFISI 312 thale cress
NP_178055    234 ALDCFFSFyvADYYPFLRGWNLQGeEAELREAVDVIARYNKMIIDEKIELWRGQnkdynraetkndvpmikDWLDILFTL 313 thale cress
NP_176122    227 ALDCFFSFnlTNYIPFLRGWNVDKaETEVREAVHIINICNDPIIQERIHLWRKKggkq----------meeDWLDILITL 296 thale cress
XP_006591735 232 LLKHVYAFsvSDYIPCLRILDLDGhKSKVKKGMRTMKKYHDPIIEKRMKQWNDGskt-----------veeDLLDVLISL 300 soybean
Q6J541       237 ILKYVYAFsiSDFVPFLRRLDLDGhRSKIMKAMRIMRKYHDPIIDDRIKQWNDGlkt-----------veeDLLDVLIKL 305 Lotus japonicus
Q6J540       237 ILKYVYAFsiSDFIPFLRRLDLDGhRSKIMKAMGIMKKYHDPIIHDRIKQWNDGlkt-----------veeDLLDVLIKL 305 Lotus japonicus
Q9M7B8       246 ALKYLYGFciSDFLPFLLGLDLDGqEKFVLDANKTIRDYQNPLIDERIQQWKSGerk-----------emeDLLDVFITL 314 cassava
Q9FLC8       234 VLTHLYAFalSDYVPWLRFLDLEGhEKVVSNAMRNVSKYNDPFVDERLMQWRNGkmk-----------epqDFLDMFIIA 302 thale cress
Q9M7B7       245 ALKYLYGFciSDYLPFLEGLDLDGqEKIVLNANKTIRDLQNPLIEERIQQWRSGerk-----------emeDLLDVFITL 313 cassava
Q43135       259 SLGLLYAFcvSDYLPWLRGLDLDGhEKIVKEANVAVNRLHDTVIDDRWRQWKSGerq-----------emeDFLDVLITL 327 sorghum
Feature 1                            #  ##  ##                                                   
O81346       313 KDEQGNPLLTADEIKPTIKELVMAAPDNPSNAVEWAMAEMVNKPEILRKAMEEIDRVVGKerlvqESDIPKLNYVKAILR 392 thale cress
NP_178055    314 KDENGKPLLTPQEITHLSVDLDVVGIDNAVNVIEWTLAEMLNQREILEKAVEEIDMVVGKerlvqESDVPNLNYVKACCR 393 thale cress
NP_176122    297 KDDQGMHLFTFDEIRAQCKEINLATIDNTMNNVEWTIAEMLNHPEILEKATNELDIIVGKdrlvqESDISQLNYIKACSK 376 thale cress
XP_006591735 301 KDVNNNPTLTLKEIKALTIELMLGGADNPSNAVECALAEMINQPYILQQATEEVDKVVGKqrmvqESDITNLNYVKACVR 380 soybean
Q6J541       306 KDANNKPLLTLKELKAQIIELAIEMVDNPSNAFEWALAEMINQPELLKRATEELDNVVGKerlvqESDIPKLQFVKACAR 385 Lotus japonicus
Q6J540       306 KDASNKPLLTLKEIKAQITELAIEMVDNPSNAFEWALAEMLNQPELLKRATEELDNVVGKerlvqESDIPKLQFVKACAR 385 Lotus japonicus
Q9M7B8       315 KDSDGNPLLTPDEIKNQIAEIMIATVDNPSNAIEWAMGEMLNQPEILKKATEELDRVVGKdrlvqESDIPNLDYVKACAR 394 cassava
Q9FLC8       303 KDTDGKPTLSDEEIKAQVTELMLATVDNPSNAAEWGMAEMINEPSIMQKAVEEIDRVVGKdrlviESDLPNLNYVKACVK 382 thale cress
Q9M7B7       314 QDSDGKPLLNPDEIKNQIAEIMIATIDNPANAVEWAMGELINQPELLAKATEELDRVVGKdrlvqESDIPNLNYVKACAR 393 cassava
Q43135       328 KDAQGNPLLTIEEVKAQSQDITFAAVDNPSNAVEWALAEMVNNPEVMAKAMEELDRVVGRerlvqESDIPKLNYVKACIR 407 sorghum
Feature 1               #   ## #                                                                 
O81346       393 EAFRLHPVaaFNLPHVALS-DTTVagYHIPKGSQVLLSRYGLGRNpk-vwADPLCFKPERHlnec----sevtLTENDLR 466 thale cress
NP_178055    394 ETLRLHPTnpFLVPHMARH-DTTLagYFIPKGSHILVSRPGVGRNpk-twDEPLIYRPERHitgn-----evvLTEPDLR 466 thale cress
NP_176122    377 ESFRLHPAn-VFMPHHVAReDTTLagYFVPKGSQILVSRLGLGRNpk-iwDEPNAFKPERYldghvekslgvtLMEPDMR 454 thale cress
XP_006591735 381 EAFRLHPVvpFNPPHVSTN-DTMVgnYFIPKGSHVLLSRHGLGRNlkvwnNEPHKFKPERHikndg---smivLSEPDLK 456 soybean
Q6J541       386 EALRLHPMeyFNVPHLCMN-DTMVgdYLFPKGTQVLLSRVALGRNpk-fwTDPLKFNPERHlkeg----idvvLTEPDLR 459 Lotus japonicus
Q6J540       386 EALRLHPMeyFNVPHLCMN-DTMVgdYLFPKGTQVLLSRVALGRNpk-fwTDPLKFNPERHlkeg----idvvLTEPDLR 459 Lotus japonicus
Q9M7B8       395 EAFRLHPVahFNVPHVAME-DTVIgdYFIPKGSWAVLSRYGLGRNpk-twSDPLKYDPERHmneg-----evvLTEHELR 467 cassava
Q9FLC8       383 EAFRLHPVapFNLPHMSTT-DTVVdgYFIPKGSHVLISRMGIGRNps-vwDKPHKFDPERHlstn----tcvdLNESDLN 456 thale cress
Q9M7B7       394 EAFRLHPVayFNVPHVAME-DAVIgdYFIPKGSWAILSRYGLGRNpk-twPDPLKYDPERHlneg-----evvLTEHDLR 466 cassava
Q43135       408 EAFRLHPVapFNVPHVALA-DTTIagYRVPKGSHVILSRTGLGRNpr-vwDEPLRFYPDRHlataa---sdvaLTENDLR 482 sorghum
Feature 1          ####  #####  ##                                                  
O81346       467 FISFSTGkRGCAAPALGTALTTMMLARLLQGFTWk-LPENETRVELMEssHDMFLAKPLVMVGDLRL 532 thale cress
NP_178055    467 LVSFGTGrRGCVGAKLGTSMIVTLLGRLLQGFDWtiPPGTTDRVELVEskENLFMANPLMACVKPRL 533 thale cress
NP_176122    455 FVTFGTGrRSCPGTKIGTSMTIMLLARLIQGFEWt-LPIGKSSVELISaeSNLFMAKPLLACAKPRL 520 thale cress
XP_006591735 457 FISFGTGrRGCPAIVLGSTMTVMLLARLVHAFSWs-APPNVSSVNLVKanNGVMLLEPLVLVAKPRL 522 soybean
Q6J541       460 FISFTTGrRSCPGVALGTTMTVMLFARMLHGFSWs-PPPDVSSIDLVPskDDLFLAKPLLLVAKPRL 525 Lotus japonicus
Q6J540       460 FISFTTGrRSCPGVTLGTTMTIMLFARMLHGFSWs-APPNVSSIDLTQssDDLFMAKPLCVVAKPRL 525 Lotus japonicus
Q9M7B8       468 FVTFSTGrRGCVASLLGSCMTTMLLARMLQCFTWt-PPANVSKIDLAEtlDELTPATPISAFAKPRL 533 cassava
Q9FLC8       457 IISFSAGrRGCMGVDIGSAMTYMLLARLIQGFTWl-PVPGKNKIDISEskNDLFMAKPLYAVATPRL 522 thale cress
Q9M7B7       467 FVTFSTGrRGCVAALLGTTMITMMLARMLQCFTWt-PPPNVTRIDLSEniDELTPATPITGFAKPRL 532 cassava
Q43135       483 FISFSTGrRGCIAASLGTAMSVMLFGRLLQGFTWs-KPAGVEAVDLSEskSDTFMATPLVLHAEPRL 548 sorghum

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