5XJC


Conserved Protein Domain Family
Slu7

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pfam11708: Slu7 
Pre-mRNA splicing Prp18-interacting factor
The spliceosome, an assembly of snRNAs (U1, U2, U4/U6, and U5) and proteins, catalyzes the excision of introns from pre-mRNAs in two successive trans-esterification reactions. Step 2 depends upon integral spliceosome constituents such as U5 snRNA and Prp8 and non-spliceosomal proteins Prp16, Slu7, Prp18, and Prp22. ATP hydrolysis by the DEAH-box enzyme Prp16 promotes a conformational change in the spliceosome that leads to protection of the 3'ss from targeted RNase H cleavage. This change, which probably reflects binding of the 3'ss PyAG in the catalytic centre of the spliceosome, requires the ordered recruitment of Slu7, Prp18, and Prp22 to the spliceosome. There is a close functional relationship between Prp8, Prp18, and Slu7, and Prp18 interacts with Slu7, so that together they recruit Prp22 to the spliceosome. Most members of the family carry a zinc-finger of the CCHC-type upstream of this domain.
Statistics
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Structure
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PubMed ReferencesClick to see Conserved Features Help

pfam11708 is the only member of the superfamily cl13203
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
5XJC_Z       161 FDYDGKRDRWNGYNPEEHM-KIVEEYAKVDLAKRTLKAQKLQe----eLAsgklveqanspkhqwgEEEPNSQMekdhns 235
CCG81311     140 TTWDSKRDRANGYDFDNHAaILKASFDRVEALRAERGQHDGV---------------------------VEDDEsnpqsv 192
EFX04902     167 LGWDAKRDRWLGFDARDYA-SMVAGREKMNEARRRLGGERG----------------------------GGGAEggeegg 217
Q7SDY6       137 LGYEAKRDVYSAYDPKQYM-EVVEEYNMLEEARRALQGDQKTp----dGE----------------GADgped------- 188 Neurospora cras...
XP_001903022 142 LGYEAKRDVWAGYDPKQYK-EVLDEYNMIEEARRKLVAETSNgedkksSE----------------EENgged------- 197
Q2H422       133 LGYEAKRDVWAAYDPKQYK-EVVEEYNLVEEARRALQGKNGD------------------------KSEgged------- 180 Chaetomium glob...
XP_011271900 122 LDYDGQRDRWYGYDSKDYK-KYLTNWE----IKQKEKENEAD-------------------------------------- 158
EER30691      79 EDYDSKRDRWYGYSTEEWL-THLKNWKQTKQPDQEIENNDSD-------------------------------------- 119
Q6BMK7        84 EDYDSKRDRWHGYEAQEWD-KIAENWDKIKKKKQKTKNASVE---------------------------EDSD------- 128 Debaryomyces ha...
Q9Y7Y2       153 VSWDAKRDRWNGYDATDYK-KVIERYEKLDELQNKGEENRDA---------------------------SENSAvkasrn 204 Schizosaccharom...
5XJC_Z       236 ED----ED----EDKY-ADDIDMPGq--nF--D-----SKRRITVRNLRIREDIAKYLRNLDPNS-----AYYDPKTRAM 292
CCG81311     193 AD------------------------------------QTKSTSNSHMRIREDTASYLAK-DREN-----DSYDPKTRAM 230
EFX04902     218 SSsktaAEgdknDTRY-EEDADMGV--------------SRTNGAADLRMREDTARYLVNLDPDS-----AKYDPKRRKV 277
Q7SDY6       189 ---------dksGFKY-DEESDMGRd--------------RATTKQSMRIREDTAKYLLNLDSDS-----AKYNPKKRAL 239 Neurospora cras...
XP_001903022 198 --------ayekGFKY-AEESDLGK---------------DRTTKKSMRIREDTAKYLLNLDSDS-----AKYNPKKRAL 248
Q2H422       181 --------vyeeGFKY-AEESELGK---------------DKTVKQSMRIREDTAKYLLNLDSDS-----AKYNPKKRAL 231 Chaetomium glob...
XP_011271900 159 ------------NTKQyDTDEEIELkelgLdhDlefinTEKQPGEKTIRIREDKAIYLQNYTNDN-----ITYDPKSRMI 221
EER30691     120 -----------------DTDYELELielgLdkKdlnknIKEDPMEKMLRDRQDIPAYIYNITSNPnnkirIDYDPKSRLA 182
Q6BMK7       129 -----------------DTDYELELvelgLdsKdiknnLKEDPLEKTIRDRQDVPAYILNITSSNk----IHYDPKSRLT 187 Debaryomyces ha...
Q9Y7Y2       205 STvsgsED-------------------------------SASITTPSLRMREDVVAYLRADNKN------LQYEPKSRSM 247 Schizosaccharom...
5XJC_Z       293 REN-PYANagKNPDE--VSYAGDN-FV-RYTGDTISMAQTQLFAWE-AYDK--------------GS------------- 339
CCG81311     231 KDAvPSAGtgIDKKD----------FT-RGSGDAAAFTKLQAFAWN-EQAT--------------GSttdeqkkkigqvp 284
EFX04902     278 VDGgAFLDesARL------FAEQN-FA-RASGEAAAFARDERYAWElHDQA--------------GDa------------ 323
Q7SDY6       240 VDA-GAI-----ADKsaALFAEES-FL-RASGEAAEFEKAQRYAWE-AQER--------------SGd------------ 284 Neurospora cras...
XP_001903022 249 VDA-GAI-----ADKsaQVFAEEA-FL-RASGEAAEFEKAQRYAWE-AQEL--------------KGd------------ 293
Q2H422       232 VDA-GAI-----GDKssQLFAEES-FL-RASGEAAEFEKAQKYAWE-AQEK--------------TGd------------ 276 Chaetomium glob...
XP_011271900 222 RDQ-St-----------GFFNEQNlFVkHMTGDALKYEKTKTFAWD-EEKK--------------GV------------- 261
EER30691     183 KDMs------------kGFLNDRSqFVkKLTGAATEFTDLQKIASGlDNEHeqerkqarlkqqffGDdsqfkne--vpet 248
Q6BMK7       188 KDPs------------kGFINDKNqFVkKLTGEAKRLDNLQKFAWEqNRQQeemkqreafeqkltGKkhseggp-deyqv 254 Debaryomyces ha...
Q9Y7Y2       248 RDEtGYHMvdDSS--------GGAgFVkASGGEKEDFEKLQMFAWE-AERS--------------GT------------- 291 Schizosaccharom...
5XJC_Z       340 --EV-HLQADPTKLELLYKSFKVKKEDFKEQQKE-------SILEKYGGQEHLDAPPAEL-LL-AQTEDYVEYSRHGTVi 407
CCG81311     285 fgDV-HAQATPSHAALLYK----ERLAAKAAEKA-------GILDRYGNDT--QALPSHLlDV-ANDEKV--YSKEGLLl 347
EFX04902     324 --GL-HLQANPTAAAHRRRVEADVEAEKRATMAA-------LMRDKYGVDE----TPAAVpSItAASEHFVEYDETGQVk 389
Q7SDY6       285 -tSL-HLQANPTAGEILRKKESEEREAKRRKRAE-------ELANQYGTQPVISDALRET--I-KESETFVEYDEAGLi- 351 Neurospora cras...
XP_001903022 294 -tSL-HLQANPTAGAFARKKETEEREAKRRKKAE-------LLASQYGEQPAVPEALKAT--I-TESETFVEYDEAGLv- 360
Q2H422       277 -tSL-HLQANPTAGALMRKKEGEEREAKRRKRAE-------LLASQYGEQPTVPEALKVA--I-TESETFVEYDEAGLi- 343 Chaetomium glob...
XP_011271900 262 --DAsNYASNPTLADIKINETKNENRIKSEKVKS-------ELMKKYDGSLSIKDQSDEE----EESEPEPQYk------ 322
EER30691     249 dlNL-SLEASPTLMMLKAKQLQKEKLKLKDKEKEkpnntknEISQKY-----------------ASSGITTNLDKn---- 306
Q6BMK7       255 dlNL-NMEANPTAMMLQARHKQEQQQASHDQKKS-------DLVAKYGGGE-----------FlNKSKEFVNVTESIQTg 315 Debaryomyces ha...
Q9Y7Y2       292 --RV-HVVANPTAGELEFRKNKASRMTTQKHIDQ-------SILDRYGDGTs-KVKDKKAkNNeKEVPDLAILDKs---- 356 Schizosaccharom...
5XJC_Z       408 -----KgqerAVACSKYEEDVKIHNHTHIWGS 434
CCG81311     348 t-------pkHVVRSRYNEDVLLNGHTSVWGS 372
EFX04902     390 erl----rapRADRSKYAEDVLPMNHTAVWGS 417
Q7SDY6       352 -----KgapkKVGKSKYLEDVYINNHTSVWGS 378 Neurospora crassa OR74A
XP_001903022 361 -----KgvprKVGRSKYPEDVFINNHTSVWGS 387
Q2H422       344 -----KgaprKAAKSKYAEDVLINNHTSVWGS 370 Chaetomium globosum CBS 148.51
XP_011271900 323 ----------DFTISKYIEDEYINNHTSVWGS 344
EER30691     307 ----------GLKKTRYPEDQLTGNHKFIYGS 328
Q6BMK7       316 dkpinKdk-nGLKRSIYPEDNYSMNHQSIWGS 346 Debaryomyces hansenii CBS767
Q9Y7Y2       357 ----------EKSKGESNTDEESENLVIVEGD 378 Schizosaccharomyces pombe 972h-
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