5NEN,5NG5,2V4D,4DNR,4TKO


Conserved Protein Domain Family
HlyD

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pfam00529: HlyD 
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HlyD membrane-fusion protein of T1SS
HlyD is a component of the prototypical alpha-haemolysin (HlyA) bacterial type I secretion system, along with the other components HlyB and TolC. HlyD and HlyB are inner-membrane proteins and specific components of the transport apparatus of alpha-haemolysin. HlyD is anchored in the cytoplasmic membrane by a single transmembrane domain and has a large periplasmic domain within the carboxy-terminal 100 amino acids, HlyB and HlyD form a stable complex that binds the recombinant protein bearing a C-terminal HlyA signal sequence and ATP in the cytoplasm. HlyD, HlyB and TolC combine to form the three-component ABC transporter complex that forms a trans-membrane channel or pore through which HlyA can be transferred directly to the extracellular medium. Cutinase has been shown to be transported effectively through this pore.
Statistics
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PSSM-Id: 425733
Aligned: 43 rows
Threshold Bit Score: 114.443
Created: 21-Aug-2024
Updated: 4-Dec-2024
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
5NEN_B        23 FTRLDKGVASPGSVTVSGNRKT-VQAPASGIIKNIA-VRDGDKVKAGEVLVQLSQVQAQAQVDSLRDQYYTTLATEGRLL 100 Serratia marces...
1_pfamImport  11 AEPLALSSELPGRIEPVRVAE--VRARVAGIVVRKR-FEEGADVKAGDLLFQIDPAPLKAAVSRAEGELARNRAVLFEAQ 87 
Q9ZNG9        43 PAPIGITSELPGRLEAYRQAE--VRARVAGIVTRRL-YEEGQDVRAGTVLFQIDPAPLKAALDISRGALPGRGQPRAAAD 119 Pseudomonas aer...
WP_004538316  47 KTSVPLSVELPGRLDAYRQAE--VRARVAGIVTART-YEEGQEVKRGAVLFRIDPAPFKAARDAAAGALEKAQAAHLAAL 123 Burkholderia ps...
P37636        43 PGSVNVLSELPGRTVPYEVAE--IRPQVGGIIIKRN-FIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNAR 119 Escherichia col...
3_pfamImport  11 AQALTLTTDLPGRTSAFRVAE--VRPQVSGILQKRS-FVEGAEVKLGQQLYQIDPRTYEAQLRRAEANRTSAQNLARRYE 87 
2V4D_E        22 AQTVTLNTELPGRTNAFRIAE--VRPQVNGIILKRL-FKEGSDVKAGQQLYQIDPATYEADYQSAQANLASTQEQAQRYK 98  Pseudomonas aer...
P37973        80 SAAVGTEILAPATVAALPGSEAvIVSRAAGAVQRVQ-RRLGDVVKAGDVLALVDSPEAAGMAAERKVAQAKADLARKTYE 158 Cupriavidus met...
Q8CWA2       101 RGPLTFSQSFPANVSYNEYQYAiVQARAAGFIDKVYpLTVGDKVQKGAPLLDLTIPDWVEAQSEYLL-LRETGGTATQTE 179 Escherichia col...
4DNR_C       101 RGPLTFAQSFPANVSYNEYQYAiVQARAAGFIDKVYpLTVGDKVQKGTPLLDLTIPDWVEAQSEYLL-LRETGGTATQTE 179 Escherichia col...
5NEN_B       101 AERDGLSIvtfspildavkdkprvaeiialqtqlfasrrqaLQSEIDGYKQSMDGIRFQLKGLQDSR-GNKQIQLSSLRE 179 Serratia marces...
1_pfamImport  88 ARVRRYEP---------------------------------LVKIQAVSQQDFDTATADLRSAEAAT-RSAQADLETARL 133
Q9ZNG9       120 KLKAYADL----------------------------------IKDRAISEREYTEAQTDARQALAQI-ASAKAELEQARL 164 Pseudomonas aer...
WP_004538316 124 DKRRRYDE---------------------------------LVRDRAVSERDHTEALADERQAKAAV-ASARAELARAQL 169 Burkholderia ps...
P37636       120 ITFNRQAS---------------------------------LLKTNYVSRQDYDTARTQLNEAEANV-TVAKAAVEQATI 165 Escherichia col...
3_pfamImport  88 TLLKTKAV-----------------------------------------------SKQQYDDALAAW-KQAEADYQVARI 119
2V4D_E        99 LLVADQAV-----------------------------------------------SKQQYADANAAY-LQSKAAVEQARI 130 Pseudomonas aer...
P37973       159 REASLFQQ---------------------------------------------------------GVtPRQEMEAAKAAL 181 Cupriavidus met...
Q8CWA2       180 GILERLRL--------------------------------------------------------AGMpEADIRRLIATQK 203 Escherichia col...
4DNR_C       180 GILERLRL--------------------------------------------------------AGMpEADIRRLIATQK 203 Escherichia col...
5NEN_B       180 QMNSMKQLAADGYLPRNRYLEVQRQFAEVNSSIDETVGRIGQLQKQLlesqqriDQRFADYQREVRTQLAQTQMDASEFR 259 Serratia marces...
1_pfamImport 134 NLGYASVTAPISGRIGRALVTEGALVGQGEATLMARIQQLDPIYADF-------TQTAAEALRLRDALKKG---TLAAGD 203
Q9ZNG9       165 RLGYATVTAPIDGRARRALVTEGALVGEDSPTPLTRVEQIDPIYVN------------FSQPAAKSRHAAGDPRRPGEGC 232 Pseudomonas aer...
WP_004538316 170 QLDYATVTAPIDGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNF-------SQPAADVESLRRAVKSGRAAGIAQQD 242 Burkholderia ps...
P37636       166 NLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDL-------TQSVQDFLR-MKEEVASGQIKQVQGS 237 Escherichia col...
3_pfamImport 120 DVQYTRVLSPISGRIGRSTVTEGALVTNGQAQSLATVTQLDPIYVDV-------TQPITKLLGLQKALESGRLQKTGENQ 192
2V4D_E       131 NLRYTKVLSPISGRIGRSAVTEGALVTNGQANAMATVQQLDPIYVDV-------TQPSTALLRLRRELASGQLERAGDNA 203 Pseudomonas aer...
P37973       182 DVAQAEALRAATVAQSAHLASDGRSVAVVSPIAGKITAQSVTLGAF--------VAPQAELFRVAGTGAVQVEAAVTAAD 253 Cupriavidus met...
Q8CWA2       204 IQTRFTLKAPIDGVI--TAFDLRAGMNIAKDNVVAKIQGMDPVWV---------TAAIPESIAWLVKDASQFTLTV---- 268 Escherichia col...
4DNR_C       204 IQTRFTLKAPIDGVI--TAFDLRAGMNIAKDNVVAKIQGMDPVWV---------TAAIPESIAWLVKDASQFTLTV---- 268 Escherichia col...
5NEN_B       260 NKLQMADFDlGNTAIT-SPVDGTVVGLNIFTqGGVVGAGDHLMDVVPsqATLVVDSRLKVDLFDKVYNGLPVDLMFTAFN 338 Serratia marces...
1_pfamImport 204 SQALTLRVEgTPYERQ-----GALQFADVAVdRGTGQIALRGKFANP--DGVLLPGMYVRVRTPQGIDNQAILVPQRAVH 276
Q9ZNG9       233 RRQGHRRAPgPGRRQR-VPLAGELLFIDLAVdPGTDTIAMRALFRNP--HRELLPGGYVQVRLQRAVNPQAITVP-DALI 308 Pseudomonas aer...
WP_004538316 243 VEVTLVRPDgSTYARK-----GKLLFADLAVdPSTDTVAMRALFPNP--ERELLPGAYVRIALDRAVARDAILVPRDALL 315 Burkholderia ps...
P37636       238 TPVQLNLENgKRYSQT-----GTLKFSDPTVdETTGSVTLRAIFPNP--NGDLLPGMYVTALVDEGSRQNVLLVPQEGVT 310 Escherichia col...
3_pfamImport 193 AEVSLTLDDgSAYPLP-----GTLKFSEVSVdPTTGSVTLRAEFPNP--NRKLLPGMFVHALLKEGVQNAAILVPQQAIS 265
2V4D_E       204 AKVSLKLEDgSQYPLE-----GRLEFSEVSVdEGTGSVTIRAVFPNP--NNELLPGMFVHAQLQEGVKQKAILAPQQGVT 276 Pseudomonas aer...
P37973       254 TSRIVAGSEaTILLANgSPLSARVQAVTPTVtGSARVATVVVVPAQP--TDRLVVGEGVQVRLRTAVADAAALSVPEDAV 331 Cupriavidus met...
Q8CWA2       269 ----PARPDkTLTIRKwTLLPG--------VdAATRTLQLRLEVDNA--DEALKPGMNAWLQLNTASEPM-LLIPSQALI 333 Escherichia col...
4DNR_C       269 ----PARPDkTLTIRKwTLLPG--------VdAATRTLQLRLEVDNA--DEALKPGMNAWLQLNTASEPM-LLIPSQALI 333 Escherichia col...
5NEN_B       339 QNKTPKIPGTVTLVSAD------RLVDKANGEPYYQMQVTVSPEGMKML 381 Serratia marcescens
1_pfamImport 277 RSSDGSAQVMVVGADERae--srSVGTGVMQGSRWQITEGLEPGDRVIV 323
Q9ZNG9       309 RT---AQSAVVKVVNPKglvedvEVRADTLQGRDWIISRGLKGGEWVIV 354 Pseudomonas aeruginosa
WP_004538316 316 RTAD-SATVKVVGQNGKir--dvTVEAAQMKGRDWIVTRGLAGGERVVV 361 Burkholderia pseudomallei
P37636       311 HNAQGKATALILDKDDVvq--lrEIEASKAIGDQWVVTSGLQAGDRVIV 357 Escherichia coli K-12
3_pfamImport 266 RDTRGVPSVWVVKADNTve--srEIQTLRTVGNAWLISNGVTEGERIIT 312
2V4D_E       277 RDLKGQATALVVNAQNKve--lrVIKADRVIGDKWLVTEGLNAGDKIIT 323 Pseudomonas aeruginosa
P37973       332 QNLDGRDVLFVRTQEGFrp---mPVLVGTRSGGSAQILSGVQAGEQVAT 377 Cupriavidus metallidurans CH34
Q8CWA2       334 DT---GNEQRVITVDADgrfvpkRVAVFQASQGVTALRSGLAEGEKVVS 379 Escherichia coli CFT073
4DNR_C       334 DT---GSEQRVITVDADgrfvpkRVAVFQASQGVTALRSGLAEGEKVVS 379 Escherichia coli K-12
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