Conserved Protein Domain Family
mut-7_like_exo
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cd06146: mut-7_like_exo 
DEDDy 3'-5' exonuclease domain of Caenorhabditis elegans mut-7 and similar proteins
The mut-7 subfamily is composed of Caenorhabditis elegans mut-7 and similar proteins found in plants and metazoans. Mut-7 is implicated in posttranscriptional gene silencing. It contains a DEDDy-type DnaQ-like 3'-5' exonuclease domain possessing three conserved sequence motifs, termed ExoI, ExoII and ExoIII, with a specific YX(3)D pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis.
Links
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Statistics
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PSSM-Id: 176655
Aligned: 18 rows
Threshold Bit Score: 197.901
Created: 14-Apr-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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Feature 1:catalytic site [active site]
Evidence:
  • Comment:The catalytic site is characterized by the presence of four negatively charged residues (DEDD) that serve as ligands for the two metal ions required for activity.
  • Comment:DEDDy exonucleases contain a conserved tyrosine at the ExoIII motif which is important in catalysis.
  • Citation:PMID 9396823
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                               # #                                       
BAB70838       96 VHLLASWEDLTRHegal------------lqcHQVVGVDVEWTPVfvag-----------------grprPSLLQVA--- 143  human
NP_197822       7 IYLVSSTDSSEFThlkw-----------sftrSTIIALDAEWKPQhsnts----------------sfptVTLLQVAcrl 59   thale cress
EAZ03725       16 VHLVTGGGSSPEHalllr----------slaaARVVALDAEWKPRrrgpavaddpsaaaaatppppqfptVTLLQVAcrg 85   Indian rice
NP_176027     356 VEDVVWVDEVNELrkats----------flegCRVVGIDCEWKPNyikgs----------------kqnkVSIMQIG--- 406  thale cress
CAL53071      372 VRWVDDVEGLRAAtdavralggsgeastsaraPPCIGLDAEWRPGdn---------------------tpVALLQIA--- 427  Ostreococcus ...
XP_001771451   30 ILFVDSPRTEEFEvfli-----------aleeAMIVAMDAEWKPVrrag-----------------vsprVSIMQIScri 81   Physcomitrell...
XP_001651037  437 VHLVDSKDKFYAMlsd-------------lcrQSMIAFDSEWKPTfgg-------------------aneVSLIQLA--- 481  yellow fever ...
XP_001749225  201 EPIFVGTEEQAAAvladvk--------adaamQRPISFDCEWAGTvsly-----------------ddtrLGIMQLA--- 252  Monosiga brev...
NP_610094     413 VNKADEFDRMLYHlq----------------qECVIYLDSEWMQSvcg-------------------dnqLCVLQIA--- 454  fruit fly
CAL52849      358 CVFVDDEKSLLEAshry------------lwnQGMIGIDTEWGADvgedadn-----------adrsgdeVAILQIA--- 411  Ostreococcus ...

Feature 1                                                                              #          
BAB70838      144 -------VEGHVFLLDVlalsqpp-tgqgaqaFSRLVAQLLSDp--------------sITKLGYGMVGDLQKLGTSCPa 201  human
NP_197822      60 s---hatDVSDVFLIDLssih--------lpsVWELLNDMFVSp--------------dVLKLGFRFKQDLVYLSSTFTq 114  thale cress
EAZ03725       86 dgdgggaAAAEVFVVDLlavp--------laeLREPLRELFERp--------------eVLKLGFRFKQDLVYLSATFAa 143  Indian rice
NP_176027     407 -------SDTKIFILDLiklyn-----daseiLDNCLSHILQSkstlklvsltedypdhKLSSGYNFQCDIKQLALSYGd 474  thale cress
CAL53071      428 -------TRGEVFLVDLlataprsagealndaTDELLQAVLWSe--------------dVYKLGFSFAYDIKRMKASYSh 486  Ostreococcus ...
XP_001771451   82 rkdefitGEEVIFVLDLlals--------aadFAFPLKTMLCSp--------------rILKLGFAFKQDQLHLSASFPg 139  Physcomitrell...
XP_001651037  482 -------TWDDVYMIDVmvsql------epldWAALAKNVFNRd--------------dVLKLSFAPSTDISMFQKALPs 534  yellow fever ...
XP_001749225  253 -------TKSAVYVIDAcaep-------gvfdFCAFATRLLQL----------------TPIIGFGVMSDRDQLKSAGVd 302  Monosiga brev...
NP_610094     455 -------TGHNVYLIDClares-----lrsehWRLLGANIFNNv--------------nIRKVGFSMVSDLSVLQRSLPl 508  fruit fly
CAL52849      412 -------SQLGVVILDVpklly-----scpdtLETTIGKMFEDe--------------eVLKLGFAVQEDLRRLSKCHKa 465  Ostreococcus ...

Feature 1                                                                                         
BAB70838      202 lahv---ekqiLGGMDLLLVHRQMrvasvpapa--------vdrarelrGLSLLVQQVLGTALDKTQQLSNWDRRPLYEE 270  human
NP_197822     115 hgceg-gfqevKQYLDITSIYNYLqhkrfgr-----------kapkdikSLAAICKEMLDISLSKELQCSDWSYRPLTEE 182  thale cress
EAZ03725      144 algssagfervEPFLDVTNAYYYLkghdmqk-----------rlpretkSLATICEELLGVYLSKELQCSDWSYRPLSEG 212  Indian rice
NP_176027     475 lkc----feryDMLLDIQNVFNEPf-----------------------gGLAGLTKKILGVSLNKTRRNSDWEQRPLSQN 527  thale cress
CAL53071      487 lkvw---sersKNLVDVKQLAFASspskm----------------slrcGLAVLTRQVTGFTLDKKEQCSDWGKRPLTEG 547  Ostreococcus ...
XP_001771451  140 peang-cfdkvEPYIDIAKLYKEFlhvnfsnlkhk----gkrfvlggthSLTAISKAVLGHPLCKDAQCSNWEQRPLSQD 214  Physcomitrell...
XP_001651037  535 fnvmy-ssqstSAILDLQLLWRHVerfdsfrfp--------yheesvnqNLANLVRLCLGKKLDKSNQFSNWAQRPLRKE 605  yellow fever ...
XP_001749225  303 ge------lvgQRVLDVMDTVAQLqqtipvpptyp----dkwqgsspriSLADLVQGLVGVSLAKHEQLADWERRPLRPE 372  Monosiga brev...
NP_610094     509 qlr----lqmpHHYLDLRNLWLELkkqrfgvelp------fgnvnragdALTDLSLACLGKKLNKSNQCSNWANRPLRRE 578  fruit fly
CAL52849      466 fk-------tvQGVVDLQKLWKLLvskartrsvsapwsteaellryqpvGLSALVAAVLGKPLDKMMRMSDWSRRPLTQS 538  Ostreococcus ...

Feature 1            #   #             
BAB70838      271 QVIYAAADAYCLLEVHQALCR 291  human
NP_197822     183 QKLYAATDAHCLLQIFDVFEA 203  thale cress
EAZ03725      213 QIQYAASDAYYLLDIFDLFHQ 233  Indian rice
NP_176027     528 QLEYAALDAAVLIHIFRHVRD 548  thale cress
CAL53071      548 QIAYAAADGHSLCLIFDKCLE 568  Ostreococcus tauri
XP_001771451  215 QILYAAADTHCLLALFDTLLS 235  Physcomitrella patens subsp. patens
XP_001651037  606 QLRYAALDAFCLLEIYDAIEK 626  yellow fever mosquito
XP_001749225  373 SLHYAALDAYVLLQVLDILSK 393  Monosiga brevicollis MX1
NP_610094     579 QILYAAIDARCLMLIYNTLIE 599  fruit fly
CAL52849      539 QMEYAALDAWTLVEVHRTLLE 559  Ostreococcus tauri

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