Conserved Protein Domain Family
MFS_FBT

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cd17484: MFS_FBT 
Folate-biopterin transporter of the Major Facilitator Superfamily of transporters
The Folate-biopterin transporter (FBT) family includes folate carriers related to those of trypanosomatids in higher plant plastids and cyanobacteria. FBT mediates folate monoglutamate transport involved in tetrahydrofolate biosynthesis. It also mediates transport of antifolates, such as methotrexate and aminopterin. The FBT family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.
Statistics
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PSSM-Id: 341037
Aligned: 36 rows
Threshold Bit Score: 271.022
Created: 12-May-2017
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative chemical substrate binding pocket [chemical binding site]
Evidence:
  • Comment:based on the structures of MFS transporters with bound substrates, substrate analogs, and/or inhibitors
  • Comment:since MFS proteins facilitate the transport of many different substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides, the residues involved in substrate binding may not be strictly conserved among superfamily members
  • Comment:the substrate binding site or translocation pore has access to both sides of the membrane in an alternating fashion through a conformational change of the MFS transporter

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 ##  ##  #                              #                            
Q9SKZ5    128 VAVAMVYFVQGVLGLARLAVSFYLKDDlhl---dpaETAVITGLSSLPWLVKPLYGFISDsvPLFGYRRRSYLVLSGLLG 204 thale cress
KFG36819   92 VISSLIAMLQGVEVLCNLAILYLYKDDfrl---dpaILTVVMGALKAPWTMKPLWAMMSDnlPLFGYRRKSYIFLGAFLC 168 Toxoplasma gondii FOU
CEM01301   50 GVVYGIALTVAVQPASRMALLYLFKNDfra---gpaVFDAFYSVMSIPWTIKPLLGLLSDsvPLGGSRRKSYGILMAAIV 126 Vitrella brassicaf...
EPR64320   47 FVIYLVGFSEGLTHLAALAIYYLLKDDlgl---sppEVSAIFIAPAIPWVFKPFFAFLSDsvAILGYRRKPYMIVFSALE 123 Toxoplasma gondii GT1
KNC35880   42 IVVYLVGLSDGLTHLASLATYYLFKDYfrl---tpyQVSLILMYPYIPFILKPVIALITDsfSIFGMRRKPYLFLFSLFQ 118 Plasmodium falcipa...
KEG00942   55 ISSSFIAMLQGVEVLCNLPILYIFKDTyql---hpaSLNILISIIKIPWAIKLLWGIISDtyPIFGYRRKYYLMFGSGLC 131 Plasmodium vinckei...
CEP03729   19 WSLYWFYFTLGVNLALPVLPTKTLLMDdlkv--epaTMAFTMSCMNLPWSVKPLYGFVSDsyPIRGRRRLPYVAFGLSIC 96  Plasmodiophora bra...
CEP01122   24 RSMCVISFTMGVTLCLPVLPVKIFLMDrarv--qpaQMAFVLSAAYAPRLLKPLFGYCSDlvPICGEHRRPYIVLGIVLN 101 Plasmodiophora bra...
SDW84311   21 ALFAIIYFAQGMSALTDQTISITFKDQgle----adDVAAFFLLASIPWFIKPLYGLISDflPLFGQRRKSYMLLTSSLA 96  Nitrosomonas oligo...
AAF64060  176 SEQIRRRPAAGPALVSWMWGTALIGIMissvilgplSDNGLAHFGVYITAAILLLSGLLFvfNLFEERRNRAARLEDAMI 255 Leishmania major
Feature 1                                         ## ###  #                                   
Q9SKZ5    205 AFSWSLMAgfvds---------kysaAFCILLGSLSVAFSDVVVDSMVVErargesq----------------svsgslQ 259 thale cress
KFG36819  169 VCATVGMGlvggf--------niwlaSGLLLLYFLGSAICNVIGEALVVEagrdass---------------eagaaktV 225 Toxoplasma gondii FOU
CEM01301  127 AVTMVAFHqvks----------plqaSCLVFASGFGMAFLTVLGQGVLVEqt-------------------------tgE 171 Vitrella brassicaf...
EPR64320  124 AVGFLMLGlspas---------vgtaVSSLFLISLSAAFCSAVAEALVVEsttgktl----------------dqsaenV 178 Toxoplasma gondii GT1
KNC35880  119 SLNFLALAflnls---------viqaSLILFFISLCASFCTTVAEALVVEssmgqty----------------sqgtnkV 173 Plasmodium falcipa...
KEG00942  132 ILSLLILGlisht--------nisltIFLLVAYFFGSALCNVIGEAQVIEagrkgsi----------------nstaknI 187 Plasmodium vinckei...
CEP03729   97 ACAALSLAkvgaas-------gllpvFLLLFLWSLALVVADAACDSLLADnvqletr----------------selgtlQ 153 Plasmodiophora bra...
CEP01122  102 VVSWSVLAllgyqs------pslaaiGVALFFEALTICLADVVVDGVIVErakreaa----------------raagglQ 159 Plasmodiophora bra...
SDW84311   97 CGAGLVAAlttqy--------nywelAILYTIMGFGLAYNDVLSDALMVEngklhg------------------ltgafQ 150 Nitrosomonas oligo...
AAF64060  256 EFLQKTKSassestagsppsphkldgHLTIDNRVEEEDDNVDEQEAAAMDaqgfvrprmdtylcgavemnrdvilrnwrM 335 Leishmania major
Feature 1        #  ##  #                                                                     
Q9SKZ5    260 SLCWGSSAFGGIVSSYFSGSLVes---------ygVRFVFGVTALLPLITSAVAVLVNEqrvvrpasgqkenitllspgf 330 thale cress
KFG36819  226 SIFFAFRKLSFAVMSYLSGVLLdy---------mdKRYVFLVATALPLSVLIANFFLVEpateefevreq---------- 286 Toxoplasma gondii FOU
CEM01301  172 SLQVSSKAWSVYYSVRYAGFGLalyyvsvaltvwrPRAVLTSLAVLPCLLFCWVLCFKErndgmlqvvpar--------- 242 Vitrella brassicaf...
EPR64320  179 SDFITAKAIGSLAVAYFSGYLLek---------tsKGSIFVATATFPLFITIVSFFMKDvttppernlka---------- 239 Toxoplasma gondii GT1
KNC35880  174 TEFIASKAIGSLSVAYFSGYFLek---------mpREYIFIATSIFPLIISLSCLFLKEkeystnrnifn---------- 234 Plasmodium falcipa...
KEG00942  188 ATFFAVRKIGFAIMHYLSGYLLsi---------mtNQHIFLISSILPLSIFISAFFVVEkrsykkrkiek---------- 248 Plasmodium vinckei...
CEP03729  154 SGVWIARSIGSLLASIFAGVAMdyf-------klsSSHMFALSGCLSLSGLASVIFIVEarappdqcsiva--------- 217 Plasmodiophora bra...
CEP01122  160 STSWMCRSAGAVVGGLVAMVGLdlf--------ehPLTIIFVTLSTTSIVSLCNVSTLCdrpgqhraaarps-------- 223 Plasmodiophora bra...
SDW84311  151 SVQWIAITTASIFVGLLGGYFAeh---------rdLHTAFAVSAIFPFTVLLMAIFFLRekkhvhsgpeafk-------- 213 Nitrosomonas oligo...
AAF64060  336 ALFCLILTLGVIANSLVSILGTwwdim---yvcivLAAVLCVSSFFTLPLAIAKAVVFMyfn------------------ 394 Leishmania major
Feature 1                               #  ##  ###  #                          #   #          
Q9SKZ5    331 lqtskqnmiqlwgaikqPNVFLPTLFIFLWQATPHSDSAMFYFttnklgFTPEFLGRVKLVTSIASLLGVGLYNGFLKtv 410 thale cress
KFG36819  287 -------fkrlisvvqqPALLHSTIFIFIMMSTPSAGSSMFYFmtnelkFGPELLGRMALFQSIASLLGILTYMWFFStv 359 Toxoplasma gondii FOU
CEM01301  243 -----eqigrlctffsfREVWGPILFIFVFVCTPTVTSPMFYFqtdtlgFTPWFIGMIDIATLVAGILGIWTYHTYFRev 317 Vitrella brassicaf...
EPR64320  240 ------qfrelseflkqSVIWGPALYIFVYMAGPDYDDALFFFftnklgFSPTFMGTLRFTYGVAALLGVLLYRFVFRqa 313 Toxoplasma gondii GT1
KNC35880  235 ------qlsdlikfintPIFLGPFLYIFVYMSGPDYDDAFFFFctnklgFRPSFMGTLRLTYGIASLIGIIIYRVFLKnc 308 Plasmodium falcipa...
KEG00942  249 ------qiksiyeiiktSYIQKLILFIFIMTSTPSSGSILFFFmanelqFTPKLLGKISMFQSIASLLAILLYMLILNki 322 Plasmodium vinckei...
CEP03729  218 ------rlrdllafwshEHIWKTCAFVFMFSAMPTSGDVIFYFmrqrlgFSLAFLGVLNVAGHASYVIGSWVYRDYLRlm 291 Plasmodiophora bra...
CEP01122  224 -----tssgdlyrfwsnGVLLRSTLFVAILSCHPSPHDAFLYFmrkklhFSTDLVGGLEVARHAAYFVTAGVFRTWLRqv 298 Plasmodiophora bra...
SDW84311  214 -----etwkalregfgeRSIWLVAAFIFLFNFSPSFGPAFFYYktdtlgFSQQYIGILSSIDSTFSIIGALIYASLSRti 288 Nitrosomonas oligo...
AAF64060  395 ---------------aiLYLSLPGVLNTFYVAKPSCLPDGPHF------SYTFYNAMNGFLGNIAGIGGTIVFTHLFPhh 453 Leishmania major
Feature 1                                             ##   #   #                        #  ## 
Q9SKZ5    411 PLRKIFLVTTIFGTGLGMtqvilvsgfnrqLGISDEWFAIGDSLILTVLAQASF-MPVLVLAARLCPEGMEATLFATLMS 489 thale cress
KFG36819  360 SLRRLLLLSTLLVTPFCLlpvvvverwnvqIGIPDTAFVVTDTVLMEFIGEFQA-MPILVLAARLCPPGLESTIYSFLLS 438 Toxoplasma gondii FOU
CEM01301  318 RFRSLYFAGTIVAFLLGTtplililrvnvrLGIPDEVFVLVDEFIMSVVAECLW-MPLMVLSGRICPDGVEATTYAMFLS 396 Vitrella brassicaf...
EPR64320  314 GFRQTLFWTIIVAVPIYVspvilvtglnqkLGISNQAFVLSGGFLIEATAEIQL-LPLLVLTASICPPGLEGSVYGMMMS 392 Toxoplasma gondii GT1
KNC35880  309 SLRKTLIITTLVSFPIYIspiiltehinkfLGISNELFVLSGGFLIEAITEIQL-LPLFILTANICQPGLEASVFATILS 387 Plasmodium falcipa...
KEG00942  323 SIQKLLLYSTIIIAPFSLlplla--vqkinIFIPNTLFIITDTILVEFVGELQS-IPILVECSRIIPEGFESTIYSFLLS 399 Plasmodium vinckei...
CEP03729  292 PFRSMLKRTIWAAFVVGIstlllvqrwnlvLHVPDWAFVLGDTAFVAACSQIAF-MPIAVLAAKVAPKGAEGTIYNSVIS 370 Plasmodiophora bra...
CEP01122  299 PIRRLVQWKLVVQGVLGLglllvl-frvvsGVVPDALFIYLDRIVWAACSEIGF-MPVVILAARLSPDGAEGTVYATFMS 376 Plasmodiophora bra...
SDW84311  289 PLRRMINIAIGLSVITLLa----------yLVYRGETSALFIHMIWGVTNMITT-LAFLDLAARACPKRAEATFFALLMS 357 Nitrosomonas oligo...
AAF64060  454 SYRFVMGLSAVLLPAASMfdvlilkrwnlaIGIPDHAMYIFGDTIIYEVCNMLLnMPMMMLMCRIAPRGSESMVFALLAS 533 Leishmania major
Feature 1      ##  #                                             
Q9SKZ5    490 ISNGGSVLGGLMGAglt--qafGITRDSFGNLSTLIILCNLSSLLPLPLLG 538 thale cress
KFG36819  439 AYNLGLGLSSLVSAglt--aayGISATNFTHLTSLIILCAATNLLPLCLIW 487 Toxoplasma gondii FOU
CEM01301  397 VNNLGMFVSSESSAlla--wllGITSHDLSNLWLMKVITLILGLSPLLILR 445 Vitrella brassicaformis CCMP3155
EPR64320  393 IRNSGSMVSRGLSAfft--wavGITATNFSHLTAYILLCGAWLLLPLFFLG 441 Toxoplasma gondii GT1
KNC35880  388 VKNLGSLTKKGTSSfit--ylmKIDSYNFDNLSLYILTCGFFLLFSLTLVP 436 Plasmodium falciparum RAJ116
KEG00942  400 VNNFAIMVSSLISSilt--yqlGITSTNFDNLPKMIVICCLTNIIPIIFLY 448 Plasmodium vinckei vinckei
CEP03729  371 VFNLGSVVSEFTGGllt--kalGVREGDFDNLWALIVICNLTTLLPVPLLS 419 Plasmodiophora brassicae
CEP01122  377 LMALGAIVSQQVSGvmm--dllGVRNDDFTFLWAMIVICSALHSLPLLFLR 425 Plasmodiophora brassicae
SDW84311  358 IFNFGTLASQNIGAqly--tslGEGSFAYTWLVAISTCTTAAIWLFVPLVH 406 Nitrosomonas oligotropha
AAF64060  534 IYHLGTSTSSAIGYllmetiwpVVTQGQCDYSNALWLVITGHIVTPVFILP 584 Leishmania major

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