4OJC


Conserved Protein Domain Family
EFF-AFF
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pfam14884: EFF-AFF 
Type I membrane glycoproteins cell-cell fusogen
EFF-AFF was first identified when EFF1 mutants were found to block cell fusion in all epidermal and vulval epithelia in the worm. However, fusion between the anchor cell and the utse syncytium that establishes a continuous uterine-vulval tube proceeds normally in eff-1 mutants and thus Aff1 was established as necessary for this and the fusion of heterologous cells in C. elegans. The transmembrane forms of FF proteins, like most viral fusogens, possess an N-terminal signal sequence followed by a long extracellular portion, a predicted transmembrane domain, and a short intracellular tail. A striking conservation in the position and number of all 16 cysteines in the extracellular portion of FF proteins from different nematode species suggests that these proteins are folded in a similar 3D structure that is essential for their fusogenic activity. C. elegans AFF-1 and EFF-1 proteins are essential for developmental cell-to-cell fusion and can merge insect cells. Thus FFs comprise an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle.
Links
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Statistics
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PSSM-Id: 464356
Aligned: 40 rows
Threshold Bit Score: 472.526
Created: 26-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
4OJC_A         48 IGLHTAVCFRLYedtqlasqeindDEnAgNqTSLLHTIRLEKLEHHHPITQRYTFGIPEVHASCICECDATSSTCTAESH 127
16_pfamImport   1 MGLHDSVCLQIS------------DNtTlTdSSLLHTIQYLRLEQHYPVIGSYKFGIPEVSSTCLCDCAGGDNICDLKKY 68 
5_pfamImport    1 MSLHDTACLSIS------------NNgT-NdTSILHTIQYLRLEQHYPIIGSYDFAIPSVSTNCICDCAGGDQICSLSSY 67 
3_pfamImport    1 MALHETTCLSLK------------VN-D-SeQSMLHTLQYLRLEQHYPITGAYRFAIPSISSKCICDCAGGEQHCNINAY 66 
PAV72324       60 LGLHESACLQLK------------TN-S-SePAFIHTLEYLRLEHHHPIAGKYSFGIPQIKPSCICDCAGGDVTCTLKEY 125
VDN08265       48 LTLKETLCLNFS---------------D-GmTMQIHTIEYVRMEQQFPVSASYKFGIPLITTNCICDCAGAEQYCSVDDY 111
VDN92734       55 LTLKETLCLNFS----------------------IHTIEYVRMEQQFPVSASYKFGIPLITTSCICDCAGAEQYCSVDDY 112
15_pfamImport   1 LTLKETLCLNFT---------------E-SpLPQMHALEFVRMEQHFPVTASYKFGIPQIKSACICDCAGAEQYCTVESY 64 
VDL76911       53 MALHEVACMTIK------------MN-N-SeSSILHTLEFLRLEQHHPVSGIYQFGIPKVTSSCICDCAGGDAPCKMSDY 118
VDN53437       52 ITLKETVCLNFT---------------S-SrTPQLHTFEFVRMEQHFPVVASYKFGIPQIHTSCICDCAGAEQYCSVETH 115 Guinea worm

4OJC_A        128 QFTACpesdksdetssCYRTFFPNQTPIGCSEDDIPKLCCDVRFKPYKNMTFLAVKLEQPTTYATFVYAAYDFVNGyWVE 207
16_pfamImport  69 HYQNCtqg------svCYRTFRPSQSSVGCFNQEKSEVCCEIKIEPFNQWKFTAIKIKQPDTVIIFRYRIFERIKQkWRD 142
5_pfamImport   68 HYKNCtvg------slCYRTYHPSQSNVGCLGSEKSEVCCDVVIEPYNNWNFKAIKVKQPDTILVFRYKIFERIRQrWRL 141
3_pfamImport   67 NYKNCstg------aiCYRTYKAAQSNVGCLSSSKSETCCEVQIDPYKDWAFQALRLNQPDTVLIFRYRIYDKVRKqWRL 140
PAV72324      126 SHGNCtha------siCYRT-HSFVSNEGCLTQGQAEACCDIAIEPYKGWRFTAIKINQPDTWLIFRYSIFSRSAQtWRQ 198
VDN08265      112 KYKNCskg------flCYRTYHSHQSSSGCLMSSKSEVCCEVIMEPYGGKSYTAVKLNQPDTVIILRHRIYERVTNhWAE 185
VDN92734      113 KY------------------------NSGCLMSSKSEVCCEVAIEPCDGRTYTAVKLTQPDTIIILRHRIYERLASrWTE 168
15_pfamImport  65 RYKNCskg------avCYRTFHSHQSNLGCISSARSDVCCEVSIEPYNEKMYTAVQLNQPDTLIVLRHRIYERIAGrWAE 138
VDL76911      119 HYKNCssg------alCYRTYHPVQTNVGCIGTQKSEACCQLQVDPYKDWTFTAIKINQPVTILVFRYSIFDRFSRrWRK 192
VDN53437      116 KYKNCskg------svCYRTYHPFQSNTGCISSSRSEVCCEIIIEPAHNKVYTAVKLNQPDTIIILKYRFLERAANrWVE 189 Guinea worm

4OJC_A        208 KDKTKIRSQLDGGTQDRHLDQK---RRISLaVTAGGRASHQLETGMYFSRTs-nGgeteELRMQ---PLNEITDNNFDRL 280
16_pfamImport 143 YSDDTIEVPLDKGIAKFELNNY---HKIEL-SITGSRPNRQLEPGLYFWQH---NepglALRGGv--TLNDIAETSMDKL 213
5_pfamImport  142 SSDDILEVPLNRGGAKFELVNY---NKIEI-TVTGGRPHRQLEPGVFFYQQf-dRfsppIVKGNi--PINDINENSLDKL 214
3_pfamImport  141 SADDVIEVPLNRGSSKFKLNNN---HRMDI-VVGGSRPHRQLEPGMYFMKEdstR----DLRGGa--PINDVFESNLDKL 210
PAV72324      199 ISSEIIKVPLNNGIARFQFNQI---HHIEL-SVSGGPTNRRVEPGMYFVREg-sH----ELHGGtltPLNDLTETHMERL 269
VDN08265      186 AASEEFEAVVNKGSAKMETIDK---RKMEI-RTTTGRAIREMYNGMYYFSNdn-----rALMMGv--RLNEPTETDIHKL 254
VDN92734      169 AASEEFEAVVNKGSAKMETIDK---RQMEI-RTTTGRAIREMLNGMYYFSNdn-----rQLMMGv--RLNEPTESDIHKL 237
15_pfamImport 139 TASEEFDVVINKGSAKIESIDRqrvRLVEI-RVTSGRAIREIYEGMYYYSDdd-----rLLMMGv--RLNEPTENDIHKL 210
VDL76911      193 ASEEVVEVPLNLGISKFDFNNV---QKIEM-VVSGSRPYRELQPGMYFLRDg-tH----ELRGSv--PVNDLGESSLEKL 261
VDN53437      190 MLSEEFEAIINKGSAKIENIDG---HKTEI-RATSGRAIREMTEGLYYFWDek-----rVLMSGv--RLNDPAESNIHKL 258 Guinea worm

4OJC_A        281 GWYRM------DDsGHFHVNNGVVKMDDIHKAKVKNCKEQTYKSILSANHYMp--------GHFNLTRPLEViKPWIQSA 346
16_pfamImport 214 GWMRY------EK-EEWSVRNGLVKLTQAQHINVLDCKNQKYISTINGEQLVvsgd-nklmESYNLGSLIIE-DPWVKAV 284
5_pfamImport  215 GWMRK------ED-GKWNVRKGQVKITQAQHINVQDCKLQRYLSTLNAEQYVsvgdgnekdENINLGNLLTD-ETWLESV 286
3_pfamImport  211 GWMRWeipagtSE-GRWDIRKGMVKVTQAHHVNVEDCKGQKYTTNFNGEQMVvsgd-egesENIDLGRQIQA-DPWIQSV 287
PAV72324      270 GWMRF------NDeGKLAIYNGDTKIKIAHSVRVINCVKQEYETKFAGREYVldtg-krvtENFDLGKALTE-ESWIKTA 341
VDN08265      255 GWLRK------NG-TSWLIRNGIIKITDSQHITVENCKEQRYLTRYNAEYFLtyg---driSDLDLGHQVDE-QNWVERA 323
VDN92734      238 GWLRK------NA-NSWLIRNGIIKITDSQHVTIENCKGQRYLTRYNAEYFItsd---driTDLDLGYPVHE-QNWIEQV 306
15_pfamImport 211 GWFRK------QD-GIWAVRNGIVKITDSQHVTVEDCKGQKYLTRYNAEYYTtds---dgiIGLDMGYRVDA-QVWVNKV 279
VDL76911      262 GWMRY------TD-GKWDIRNGQVKIKQAHHVNVENCKQQLHKSTLNGEQLVyvsg-devvEEFDLGRALTS-DPWIETA 332
VDN53437      259 GWLRR------EE-GIWVIRNGIIKITDSQHITIENCKSQRYLTRYNADYFLtds---ndiSQMDLGFRVDE-LSWVERV 327 Guinea worm

4OJC_A        347 RIfdsslRQAVVTHAEGTNLQISIHldDEvesQNLVFFHNASRIRDFSGSIIVDSKSNRLFNLTVYEASGKIDGSVKMST 426
16_pfamImport 285 SVid--sRFVKIEHGEGTGIVGSIK--TE---FKPLMLTHSSQFESFNGTLQLDKYSNRFMNVSFFEAKGTLIGTIFSSE 357
5_pfamImport  287 SVke-dgRVMKIVHSEGIGILGSVK--TE---FKPLMLTHSSQFASFNGTIQLDKESNRFLNITFHEAKGTVIGMVYSSE 360
3_pfamImport  288 DVg---tRVVKVEHAEGTNILISLT--TV---SRPQILHHSSQFSAFNGSVQVDRESNRFLNITFQEAKGTLIGQLYSSE 359
PAV72324      342 RVe---sRVVVVEHGEGSYVNIDVL--TE---TRPQILKHPSQMQNFSGLIQLDRESNRFLNVTFFGVKGTLIGKIYKSE 413
VDN08265      324 EIsg-ddRAVRIVHAEGSVIRVSVS--SG---ARQ-------------------------------GGKGTLIGYVYRSD 366
VDN92734      307 EIln-dnRAVRIVHAEGTVVHISVS--SD---TRPIIVRHASHLLTFNGTIRMDDQSNRFLNLTILGGKGTLIGYVHRSE 380
15_pfamImport 280 SVaa-dgRSVRIIHGEGTAVHLTVS--SE---TRPLIVQFSSQVQSFSGAIQMDENSNRFLNLTIQGGKGTLIGYVHQSE 353
VDL76911      333 VYe---gRFVRVEHAEGTTISVHMT--SE---IRPHMLRHISQLESFDGMIQVDRDSNRYLNVSFTGSKGTLLGSIFSSE 404
VDN53437      328 LIss-ntRSIRVLHAEGTVVHLTIT--TD---KKPLIVQHTSQIRSFDGFLRMDDKSNRFLNLSLIDVKGTLIGYIHQSE 401 Guinea worm

4OJC_A        427 GFGSDtIHTFTAYVsdlhasNRSMIIpLPAIVGQGARAICLRADSMaDiDKICHVIEYFESPLEIDLVEGKWHEMIGTCP 506
16_pfamImport 358 AKDRG-DLSFSVQIdgtstrDYNFNV-AVPPSVNGSRYVCVHPSGDyM-GQHCVWMTFNEVPLETFTISNHWISDQAECH 434
5_pfamImport  361 NKANI-DIVFSVLIdgtstrEFNCII-TVPGSVNSSRYVCFHPAGDvR-GQQCSWLQYKSVPIPVYTVPHRWVSGKGDCE 437
3_pfamImport  360 ERQHM-DIVFSVQIdgpttrSFNAIV-SIPSSVNSRRYICFHPAGDvD-GQMCKWFEFKEDPLPKYKVAHRWQTGPADCA 436
PAV72324      414 TKTEP-EMTFSVQTslsktlDQLSII-SVPASINGKRYVCFYPASDlK-GEICKWFNYDSQPLHSYKALNPFEMQVGDCH 490
VDN08265      367 DKSTT-DWSFSIEIgtatrtKFMATVgGIPSGIISDRYVCLQPAGDaN-AEQCKWLKYEASPLRERHMAHRWQAGIGNCP 444
VDN92734      381 DKSST-DWSFSIEIgtatrtKFTATIgGIPTGIISDRYVCLQPAGDaS-AEQCKWLKYEASPLRERHVAHRWQAGIDNCP 458
15_pfamImport 354 DKSIT-EWSFSVELgasirqHFRTTVgGIPPDINSDRYVCMHPSGDiF-AEICKWLRYDSRPLRERHVAHHWKTNIGECP 431
VDL76911      405 KKDQI-DMAFSVLVds---kNQRSII-SIPSTINSSRFVCFHPSGDvE-SEICKWFRYETQRLNSYRVAHRWATGRGDCA 478
VDN53437      402 EKTKT-EWSFSVEVgsflkhHFITTIgGIPPEINNDRYVCIHPAGDiN-AEKCKWLQYEADPLRQVRYTPRWQIGIGDCP 479 Guinea worm

4OJC_A        507 TCNQINFNGMMKFLNPAHWIK 527
16_pfamImport 435 GCNEFTIESIIEKLDPQKMWK 455
5_pfamImport  438 NCNERGVDYFTAKLDPRTWFK 458
3_pfamImport  437 GCNEIGVENFIEKMDPRKWLD 457
PAV72324      491 GCNARGLEGYLVSLDPRSWLN 511
VDN08265      445 GCNERGIENFLLKLDPRQWLD 465
VDN92734      459 GCNERGIENFLLNLDPRQWLN 479
15_pfamImport 432 GCNERGFDNFLQKLDPRQWFD 452
VDL76911      479 GCNERGVEHFLTSLDPRQWFN 499
VDN53437      480 GCNERGFDNFLQKLDPRQWLN 500 Guinea worm
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