FH DAY35S

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Result Filters

Organism: Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus

Attributes

strain	C57BL6
sex	Male
tissue	Equal amounts of pooled ventral prostate, dorsolateral prostate, and coagulating gland (anterior prostate) from five 35 day old male mice, subtracted
lab host	BM25.8
tissue	prostate
vector	pTriplEx2
v_type	plasmid (ampicillin resistant)
re_1	SfiI
re_2	SfiI

Description: Library was constructed using Clontechs SMART cDNA library construction kit. 0.5ug of total RNA from pooled 35-day dorsolateral prostate, 0.5ug of total RNA from pooled 35-day ventral prostate, and 0.5ug of total RNA from pooled 35-day coagulating gland (anterior prostate ) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 21 cycles of PCR. One prominent band from the double stranded cDNA was removed from the gel and the remaining cDNA was gel isolated. This subtracted cDNA was digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate from five 35 day old male C57BL6 mice by Colin Pritchard.