BioSample

In the context of the EU-funded project REGIA (QLG-CT11999-00876, coordinator Javier Paz-Ares), a set of transcription factor ORFs was generated. The ORFs were produced in a decentralized way in the labs of the participants. Most of the ORFs were generated by RT-PCR using cDNA from various A. thaliana tissues as a template. Initially, it was planned to use yeast recombination to move the ORFs from the cloning vectors into target constructs. For this reason, a number of the clones contain 'REGIA tags' (RG_tag1: 5pr-AATTCCAGCTCACCACC-3pr; RG_tag2: 5pr-CATGGCAATTCCCGGGGATC-3pr). During the lifetime of the project, the GATEWAY system became available and finally all ORFs were transferred into GATEWAY vectors. At the end of the REGIA project, the clones were collected as plasmid DNA. For end-sequencing at the DNA core facility of the MPI of Plant Breeding Research (ADIS; head: Bernd Weisshaar), the plasmids were re-transformed into DH5alpha, arrayed into 96-well plates and new plasmid DNA was prepared. Re-transformation proved to be difficult because a number of clones displayed poor growth. Data submission has been handled by GabiPD (http://gabi.rzpd.de).