Exelixis piggyBac WH insertions

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Result Filters

Organism: Drosophila melanogaster (fruit fly)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Ephydroidea; Drosophilidae; Drosophilinae; Drosophilini; Drosophila; Sophophora; melanogaster group; melanogaster subgroup

Attributes

strain	isogenic w- strain
vector	piggyBac WH (GenBank accession number AY515148)
v_type	piggyBac transposable element

Description: An isogenic w- Drosophila melanogaster strain was mutagenized by remobilization of transposable elements. We remobilized the WH element using the constitutive alpha-1 tubulin:piggyBac transposase source. We remobilized the WH element from a single ammunition element on the Binsinscy balancer chromosome in dysgenic females. We outcrossed dysgenic virgin females in vials to the isogenic w- strain and selected new hops in the following generation. All lines were mapped to a chromosome by standard genetic methods, examined for homozygous viability, and used for recovery of flanking genomic sequence by inverse PCR.