Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction ,ligated with EcoR I adaptor , digested with NotI and then cloned directionally into pYX-Asc vector . The library tag sequence located between the Not I site and the polyA tail is TTATTGAAGT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin , Ph.D. , program coordinator.
Nucleotide
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on