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Tissue was collected from two pooled placentas from the 21st day of pregnancy. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.25 kb fragments for an average insert size of 2.05 kb. A normalized version of this library is also available (NIH_MGC_270). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Mammalian Gene Collection library
Nucleotide
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