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NIH_MGC_248

Identifiers
BioSample: SAMN00175241; EST: LIBEST_016038
Organism
Rattus norvegicus (Norway rat)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Attributes
lab hostDH10B TonA
tissuespleen
vectorpExpress-1
v_typeplasmid
re_1EcoRV
re_2NotI
Description

RNA obtained from testis tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.7 kb. This is a primary library (normalized library is NIH_MGC_249) and was constructed by Open Biosytems. Note: this is a NIH_MGC library

Submission
National Cancer Institute / NIH, Daniela S. Gerhard, Ph.D.; 2004-07-14
Accession:
SAMN00175241
ID:
175241

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