BioSample

Full length cDNA was synthesized from adult testis poly(A)+ RNA with ZAP-cDNA Synthesis Kit (Stratagene). Size-fractionated cDNA (mainly 0.5-3kb in length) were directionally cloned into Uni-ZAP XR vector. The cDNA clones were excised from Uni-ZAP vector using ExAssist helper phage. Excised pBluescript phagemids were used to transform E coli XL1-Blue cells. The plasmid library was normalized by using a reassociation-kinetics-based approach according to Bonaldo et al., (Genome Research 6: 791-806,1996) with minor modifications. The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.