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About 1 week after bolting, cDNA synthesis using SuperscriptTM-system (Invitrogen) with an oligo(dT)-primer containing NotI restriction site and a SalI adapter. The main library (plate numbers begin with 1) of 38,000 clones was rearrayed into the sublibrary (plate numbers begin with 201) containing 5,000 putative expression clones. Average insert size is 1 kb. Note: The rearrayed sublibrary (plate numbers begin with 201) was sequenced. Library generation and sequencing was granted in context of GABI-LAPP; data are also accessible at https://gabi.rzpd.de
Nucleotide
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