BioSample

Subtractive library constructed using the CLONTECH PCR-select cDNA subtraction kit. Transgenic plants homozygous for the ag-1 mutation and carrying a 35S:AG-GR construct were generated. RNA was isolated from inflorescences with floral meristems and unopened floral buds and without open or older flowers from transgenic plants that were treated with dexamethasone for 3 days, and used for synthesis of the tester cDNA. RNA from the inflorescences of transgenic plants treated with a control solution without dexamethasone was used to synthesize the driver cDNA. It was observed that a 3-day treatment with dexamethasone can induce anther and carpel characteristics in the flowers of the ag-1 35S:AG-GR plants.