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Barstead mouse pooled organs MPLRB4

Identifiers
BioSample: SAMN00155211; EST: LIBEST_000609
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
strainFVB/N
sexmixed
tissuepooled organs
development stage7 day
lab hostDH10B
tissuepooled
vectorpT7T3D-PacI
v_typephagemid (ampicillin resistant)
re_1EcoRI
re_2NotI
Description

1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [GTTGGATTCGGTACC], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

Submission
Washington University School of MedicineP, Marra M/Mouse EST Project; 1997-02-10
Accession:
SAMN00155211
ID:
155211

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