BioSample

Priming method: Sfi-(dT)30 Primed ; Priming sequence: 5.ATTCTAGAGGCCGAGGCGGCCGACATG(T)30VN ; Directionally cloned, 5' cloning site: Sfi A site GGCCATTACGGCC ; 5' linker/adaptor sequence: 5.AAGCAGTGGTATCAACGCAGAGTGGCC ; 3' cloning site: Sfi B site GGCCGAGGCGGCC ; 3' linker/adaptor sequence: same as the priming sequence ; Average insert size: 2kb ; For PCR insert analysis: Use M13 Forward and reverse primers ; Library Amplified ; Recombinants (inserts): 98% ; Library complexity: 5x106 ; Full-length construction (method): SMART, a Clontech method The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Low intensity clones were selected as they represented rare expressed clones. The hybridization intensities for all clones span from 0 to 1.8 million counts and the low abundant class ranged from 0 to 13,000.