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Schiller mouse MAC13

Identifiers
BioSample: SAMN00155988; EST: LIBEST_001409
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
tissuecolon cancer
cell linecolon cancer cell line MAC13
lab hostSOLR
vectorpBluescript SK- (Stratagene)
v_typeplasmid (ampicillin resistant)
re_1EcoRI
re_2XhoI
Description

Double-stranded cDNA was prepared from cell line MAC13 using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

Submission
Washington University School of MedicineP, Marra M/Mouse EST Project; 1998-11-16
Accession:
SAMN00155988
ID:
155988

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