BioSample

Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two male schizophrenics, ages 30 and 32 (S-30, S-121) subtracted by pool of two male bipolars, age 34 and 48 (S-75, S-83). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, [email protected]].