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MPIZ-ADIS-013

Identifiers
BioSample: SAMN00172234; EST: LIBEST_012686
Organism
Arabidopsis thaliana (thale cress)
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Attributes
cultivarNiederzens-1 (Nd-1)
tissuewhole plant
development stageadult plant, mixed stresses
lab hostE. coli TOP10
vectorpSPORT1
re_1SalI
re_2NotI
Description

RNA from total, adult, 6 weeks old Arabidopsis thaliana (accession Nd-1) plants, grown in the greenhouse under long day conditions in soil treated for 24 hours with different stresses, (1) at 4 Grad C in the dark, (2), at 37 Grad C in the dark, (3) lying in the lab after removing from soil, (4) in the greenhouse after wounding with a foreceps, (5) in the lab watering with a 150 mM NaCl solution, (6) at 26 Grad C in the light/UV; equal quantities of stressed plant material were pooled; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites SalI-NotI, primer sites and orientation: T7-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-SP6; Note: Sequencing granted in the context of the GABI Arabidopsis Verbund I: Genetic Diversity, 'Establishment of high-efficiency SNP-based mapping tools and development of methods for genome-wide mutation detection' PI: Bernd Weisshaar Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de. This clone is available from RZPD; contact RZPD ([email protected]) for further information.

Submission
Bielefeld University, Weisshaar B; 2003-02-19
Accession:
SAMN00172234
ID:
172234

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