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5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT (30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.67 kb (range 0.8-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).
Nucleotide
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