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Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with spores of Peronospora parasitica strain Emwa, and tissue harvested 12h, 1, 2 and 3 days after infection ; driver = plants sprayed with water and tissue harvested 12h, 1, 2 and 3 days. Cloned into Advantage PCR cloning vector (Clontech).
Nucleotide
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