MPZFRiken1

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Result Filters

Organism: Danio rerio (zebrafish)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi; Cypriniphysae; Cypriniformes; Cyprinoidei; Danionidae; Danioninae; Danio

Attributes

tissue	intestine (2 samples) and liver (pooled)
lab host	DH10B (phage-resistant)
vector	pBluescriptR
v_type	plasmid (ampicillin resistant)
re_1	XhoI
re_2	BamHI

Description: Oligo-dT primed cDNA was produced from intestine (two independent samples) and liver using 5'-GAGAGAGAGAAGGATCCAAXXXXXXTTTTTTTTTTTTTTTTVN-3' with the following tags substituting for XXXXXX: CAAGAG (tag C04): liver; CGGTAT (tag C12): intestine; and CGTATG (tag D01): intestine. cDNA was pooled and size- selected for a 1.6 kb average insert. Library was constructed using the Cap- Trapper method as described in Genomics 2001: 77(1-2)79-90. Library donated by M. Pack, M.D. (University of Pennsylvania School of Medicine).