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Total RNA was extracted from shock-frozen tissue; mRNA was purified on Ambion's poly(A)Purist columns. cDNAS was synthesised by the oligo-capping method described by Maruyama, K. and Sugano, S. (1994) Gene 138: 171-174 and by Suzuki, Y. et al. (1997) Gene 200: 149-156 in order to enrich full-length cDNAs. The molecules were the inserted into the pDONR222.1 (Invitrogen), cloned and sequenced at the Genoscope, Evry, France by Applied Biosystems 3730 automated sequencers.
Nucleotide
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