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A cDNA library enriched for Arabidopsis genes differentially expressed during disease (in response to infection by P. syringae pv. tomato DC3000) was constructed using CLONTECH PCR-Select cDNA Subtraction Kit (CLONTECH Laboratories, Inc.). 'Tester'= leaf tissue from plants infiltrated with 5 x 107 cfu/mL of Pst DC3000, 'Driver' = leaf tissue from plants infiltrated with 10 mM MgCl2. cDNA fragments were cloned in PCR 2.1 TOPO vector
Nucleotide
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