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Metagenome-assembled genome: SRR19759419_bin.49_metawrap_v1.3_MAG

Identifiers
BioSample: SAMEA112223248; SRA: ERS14334047
Organism
Muribaculum intestinale
cellular organisms; Bacteria; Pseudomonadati; FCB group; Bacteroidota/Chlorobiota group; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; Muribaculum
Attributes
broad-scale environmental contextmouse digestive system
collection datenot provided
environmental mediumfeces
geographic locationnot provided
investigation typemetagenome-assembled genome
isolation sourcemouse gut metagenome
project nameA growing body of evidence supports the notion that the gut microbiome plays an important role in cancer immunity. However, the underpinning mechanisms remain to be fully elucidated. One attractive hypothesis envisages that among the T cells elicited by the plethora of microbiome proteins a few exist that incidentally recognize neo-epitopes arising from cancer mutations (molecular mimicry (MM) hypothesis). To support MM, the human probiotic Escherichia coli Nissle was engineered with the SIINFEKL epitope (OVA-E.coli Nissle) and orally administered to C57BL6 mice. The treatment with OVA-E.coli Nissle, but not with wild type E. coli Nissle, induced OVA-specific CD8+ T cells and inhibited the growth of tumors in mice challenged with B16F10 melanoma cells expressing OVA. The microbiome shotgun sequencing and the sequencing of TCRs from T cells recovered from both lamina propria and tumors provide evidence that the main mechanism of tumor inhibition is mediated by the elicitation at the intestinal site of cross-reacting T cells, which subsequently reach the tumor environment. Importantly, the administration of Outer Membrane Vesicles (OMVs) from engineered E. coli Nissle, as well as from E. coli BL21(DE3)DompA, carrying cancer-specific T cell epitopes also elicited epitope-specific T cells in the intestine and inhibited tumor growth. Overall, our data strengthen the important role of MM in tumor immunity and assign a novel function of OMVs in host-pathogen interaction. Moreover, our results pave the way to the exploitation of probiotics and OMVs engineered with tumor specific-antigens as personalized mucosal cancer vaccines.
sample nameSRR19759419_bin.49_metawrap_v1.3_MAG
ENA-CHECKLISTERC000047
ENA-FIRST-PUBLIC2022-12-13
ENA-LAST-UPDATE2022-12-13
External IdSAMEA112223248
INSDC center aliasEMG
INSDC center nameEMG
INSDC first public2022-12-13T16:27:12Z
INSDC last update2022-12-13T16:27:12Z
INSDC statuspublic
Submitter IdSRR19759419_bin.49_metawrap_v1.3_MAG
assembly qualityMany fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly softwaremetaSPAdes v3.15.3
binning parametersMaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
binning softwaremetawrap v1.3
broker nameEMG broker account, EMBL-EBI
completeness score98.3
completeness softwareCheckM
contamination score0.0
geographic location (latitude)not provided
geographic location (longitude)not provided
local environmental contextdigestive tube
metagenomic sourcemouse gut metagenome
sample derived fromSAMN29213142
sequencing methodIllumina NovaSeq 6000
taxonomic classificationThe taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Muribaculaceae;g__Muribaculum;s__Muribaculum intestinale
taxonomic identity markermulti-marker approach
Description

This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19759419 of study SRP382781.

BioProject
PRJEB55801
Retrieve all samples from this project

Submission
EBI; 2022-12-14
Accession:
SAMEA112223248
ID:
32220421

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