| broad-scale environmental context | mouse digestive system |
|---|
| collection date | 2019-11-18 |
|---|
| environmental medium | feces |
|---|
| geographic location | China |
|---|
| investigation type | metagenome-assembled genome |
|---|
| isolation source | mouse gut metagenome |
|---|
| project name | To evaluate the role of probiotics in restoring the antibiotic-destroyed gut microbiota and efficacy of ICB therapies, we studied the effect of Lactobacillus rhamnosus Probio-M9 combined with anti-PD-1 on mouse colon cancer and the dynamic changes of gut microbiome by metagenome analyses. The results showed that the combination of Probio-M9 significantly improved tumor inhibition compared with ICB therapy alone in antibiotics treated mice, which could be attribute to the upregulated microbial diversity and increased abundance of beneficial bacteria such as Bifidobacterium pseudolongum, Parabacteroides distasonis and some species of Bacteroides after Probio-M9 supplemented. These changes of structure and composition in gut microbiota caused the alternation of microbiome function, of which the sugar degradation, vitamin and amino acid synthesis pathways were significantly enriched. Collectively, this study indicated that Probio-M9 enhanced the host's immunotherapeutic response by promoting the colonization of beneficial bacteria and improving intestinal function. |
|---|
| sample name | SRR15604559_bin.10_metawrap_v1.3_MAG |
|---|
| ENA-CHECKLIST | ERC000047 |
|---|
| ENA-FIRST-PUBLIC | 2023-01-21 |
|---|
| ENA-LAST-UPDATE | 2023-01-21 |
|---|
| External Id | SAMEA112229335 |
|---|
| INSDC center alias | EMG |
|---|
| INSDC center name | EMG |
|---|
| INSDC first public | 2023-01-21T00:26:27Z |
|---|
| INSDC last update | 2023-01-21T00:26:27Z |
|---|
| INSDC status | public |
|---|
| Submitter Id | SRR15604559_bin.10_metawrap_v1.3_MAG |
|---|
| assembly quality | Many fragments with little to no review of assembly other than reporting of standard assembly statistics |
|---|
| assembly software | not provided |
|---|
| binning parameters | MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters. |
|---|
| binning software | metawrap v1.3 |
|---|
| broker name | EMG broker account, EMBL-EBI |
|---|
| completeness score | 94.59 |
|---|
| completeness software | CheckM |
|---|
| contamination score | 0.0 |
|---|
| geographic location (latitude) | 40.51 |
|---|
| geographic location (longitude) | 110.7 |
|---|
| local environmental context | digestive tube |
|---|
| metagenomic source | mouse gut metagenome |
|---|
| sample derived from | SAMN20968412 |
|---|
| sequencing method | Illumina HiSeq 2500 |
|---|
| taxonomic classification | The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__ |
|---|
| taxonomic identity marker | multi-marker approach |
|---|