RP D melanogaster mixed embryo RACE from pooled PC... - BioSample

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Result Filters

Organism: Drosophila melanogaster (fruit fly)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Ephydroidea; Drosophilidae; Drosophilinae; Drosophilini; Drosophila; Sophophora; melanogaster group; melanogaster subgroup

Attributes

Description: Sequences of RNA Ligase Mediated Rapid Amplification of cDNA Ends (RLM-RACE) products representing 5' ends of selected transcripts. Total RNA isolated from mixed stage embryos of the 'iso-1' isogenic strain was provided by the laboratory of Peter Cherbas (University of Indiana, Bloomington). Gene-specific 5' RLM-RACE products were generated using the FirstChoice RLM-RACE procedure (Ambion). Normalized pools of RACE products were created and either cloned and sequenced with an ABI3730 or directly sequenced on a 454 Life Science sequencer. Vector sequences were removed and the RNA adapter sequence was used to determine the orientation of the clone and was removed from the sequence. Each sequence represents a potential transcription start site and is oriented along the direction of transcription. NOTE: clones are not preserved and are not available for distribution.