Microbe sample from Pseudomonas sp.

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Result Filters

Organism: Pseudomonas sp. CES
cellular organisms; Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Attributes

strain	CES
isolation source	coffee ground-enriched garden soil
collection date	2007
geographic location	USA: Iowa, University of Iowa, Iowa City
sample type	cell culture
biomaterial provider	Mani Subramanian lab, University of Iowa
collected by	University of Iowa Center for Biocatalysis and Bioprocessing
identified by	Chi Li Yu

Description: The CES strain was isolated from soil at the University of Iowa Center for Biocatalysis and Bioprocessing using an enrichment procedure with caffeine as sole carbon and nitrogen source. Prior to isolation, coffee grounds had been amended to the soil for ten days. The soil was then collected and incubated in M9 minimum medium with .25 percent caffeine as described previously previously (Yu CL, Louie TM, Summers R, Kale Y, Gopishetty S, Subramanian M. J Bacteriol. 2009 Jul;191(14):4624-32. Epub 2009 May 15.) The strain was initially characterized as Pseudomonas sp. Strain CES (for Coffee Enriched Soil) based on 16S rRNA sequencing and FAME analysis. CES uses the N-demethylation pathway for caffeine breakdown.