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The UI-R-DO1 library is a normalized Rat Cell Line R3327-5P library (nR5P) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996.) The oligonucleotide used to prime first strand synthesis contained the sequence tag CACGTGAGAT between the Not I cloning site and dT18 stretch. The rat cell line R3327-5P was provided by Mary Hendrix of the University of Iowa.
Nucleotide
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