CK Drosophila melanogaster embryo BlueScript

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Result Filters

Organism: Drosophila melanogaster (fruit fly)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Ephydroidea; Drosophilidae; Drosophilinae; Drosophilini; Drosophila; Sophophora; melanogaster group; melanogaster subgroup

Attributes

Description: mRNA purified from rough endoplasmic reticulum-bound polysomes was used as a template. cDNA's directionally cloned at HinDIII and PstI in Bluescript SKt. Cloned into HinDIII, but the site was destroyed to add an adapter sequence. So ClaI is now the restriction enzyme at site I of vector. Primers - 5' universal, 3' m13-20 (reverse), 3' T3, 5' T7.