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RACE-PCR was performed with the Marathon(TM) cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Total RNA was extracted from the leaves of about four-week old wild-type Arabidopsis Col-0 plants using the TRIzol procedure (Invitrogen, Carlsbad, CA). Template mRNA was then purified from total RNA using Dynabeads Oligo (dT)25 (Dynal Biotech, Lake Success, NY, USA). RACE-PCR products were cloned into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA) and sequenced.
Nucleotide
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