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The library was prepared by Catherine S. Lee and has not been published. The pancreas was obtained from Gerard Gradwohl (PNAS 97 P1607-1611, 2000). The cDNA's were prepared with an oligo containing a NotI site, and SalI linkers were added to the ends. The inserts were cut with NotI before being cloned into the NotI-SalI sites in the vectors. This is one of two libraries, ngn3 wt and ngn3 -/-. The wt library is in pSPORT1, T7 promoter is 5'.
Nucleotide
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