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FDR103

Identifiers
BioSample: SAMN00152483; EST: LIBEST_020694
Organism
Danio rerio (zebrafish)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi; Cypriniphysae; Cypriniformes; Cyprinoidei; Danionidae; Danioninae; Danio
Attributes
strainTubingen (TU)
sexMales and females mixed
tissueBrain (including tissues and cartilage around brain)
development stageAdult fish of 3-4 months old
lab hostEscherichia Top10
tissueBrain
vectorpGIS4a
v_typePlasmid
re_1BseRI
re_2Not I
Description

Priming method: GsuI-d(T) priming ; Priming sequence: 5' GAGCTAGTTCTGGAG(T)16VN ; Directionally cloned, 5' cloning site: NotI site GCGGCCGC ; 5' linker/adaptor sequence: 5' GGATCCGAC 3' ; 3' cloning site: BseRI ; Insert size range: 2.5kbp-700bp ; For PCR insert analysis: M13 forward and reverse primers ; Library is not amplified ; Recombinants (inserts): 94% ; Library complexity: 6.38 X 106 cfu ; Full-length construction (method): Ng et al, Nature Method 2005 2(2):105-111 The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Low abundant clones were selected by the Colony Array Subtractive Hybridization (CASH) analysis as they represented less abundantly expressed clones. The hybridization intensities for all clones span from 0 to 1 million counts and the low abundant selected clones in this library ranges from 0 to 45,000.

Submission
Genome Institute of Singapore, C. Wei; 2006-12-12
Accession:
SAMN00152483
ID:
152483

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