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Elicited cell culture

Identifiers
BioSample: SAMN00161793; EST: LIBEST_007263
Organism
Medicago truncatula (barrel medic)
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago
Attributes
tissueCell cultures derived from root tissues
development stageCell suspensions were subcultured every 14 days. Cells were induced six days after subculture
vectorLambda Zap
v_typePlasmid
Description

Cells were induced with yeast cell wall extracts equivalent to 50ug/ml glucose in the final concentration. Samples were taken at 0.5, 1, 12 and 24 hours after induction. Equal amounts of RNA from each time point were pooled and used for mRNA isolation.

Submission
The Samuel Roberts Noble Foundation, Dixon RA; 2000-12-19
Accession:
SAMN00161793
ID:
161793

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