BioSample

This cDNA library was constructed from mRNA isolated from immature leaves (unfurled trifoliate) of greenhouse grown plants that were 2 week old. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restrictions site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. This library was constructed in the laboratory of Dr. Lila Vodkin by Anu Khanna at the University of Illinois at Urbana-Champaign (email [email protected])