MIGS Cultured Bacterial/Archaeal sample from Rhizo... - BioSample

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Identifiers: BioSample: SAMN05251099; Sample name: Root nodule isolate Rhizobium leguminosarum bv. trifolii Rt24.2 from Trifolium pratense growing in Poland

Organism: Rhizobium leguminosarum bv. trifolii
cellular organisms; Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; Rhizobium leguminosarum

Attributes

strain	Rt24.2
collection date	1986-09-10
broad-scale environmental context	field
local-scale environmental context	clover root nodule
environmental medium	soil
geographic location	Poland: Pulawy
host	Trifolium pratense
isolation and growth condition	19020864
latitude and longitude	51.43 N 21.98 E
number of replicons	4
reference for biomaterial	Skorupska et al. J Plant Physiol (1995) 147:93-100
air temperature regimen	degrees Celsius 28
observed biotic relationship	symbiont
depth	0.2 m
elevation	115 m
estimated size	7,653,217 bp
extrachromosomal elements	3 megaplasmids, among them one symbiotic plasmid
host age	6 months
host dry mass	1 g
host length	0.3 m
host taxonomy ID	57577 (57577)
host total mass	11 g
host wet mass	11 g
isolation source	field clover plant
sample collection device or method	isolation of symbiotic bacterium from clover root nodule
sample material processing	bacterial growth using media specific for rhizobia
sample size	crushed root nodules suspended in 1 ml of sterile water
sample storage temperature	room temperature
sample volume or weight for DNA extraction	10 g
source material identifiers	Tp-Rt24.2
subspecific genetic lineage	biovar trifolii
temperature	18 degrees C
tissue culture growth media	root nodules, field growth
trophic level	diazotroph
biovar	trifolii