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Metagenome-assembled genome: SRR15604638_bin.75_metawrap_v1.3_MAG

Identifiers
BioSample: SAMEA112229629; SRA: ERS14340528
Organism
uncultured Clostridia bacterium
cellular organisms; Bacteria; Bacillati; Bacillota; Clostridia; environmental samples
Attributes
broad-scale environmental contextmouse digestive system
collection date2019-11-18
environmental mediumfeces
geographic locationChina
investigation typemetagenome-assembled genome
isolation sourcemouse gut metagenome
project nameTo evaluate the role of probiotics in restoring the antibiotic-destroyed gut microbiota and efficacy of ICB therapies, we studied the effect of Lactobacillus rhamnosus Probio-M9 combined with anti-PD-1 on mouse colon cancer and the dynamic changes of gut microbiome by metagenome analyses. The results showed that the combination of Probio-M9 significantly improved tumor inhibition compared with ICB therapy alone in antibiotics treated mice, which could be attribute to the upregulated microbial diversity and increased abundance of beneficial bacteria such as Bifidobacterium pseudolongum, Parabacteroides distasonis and some species of Bacteroides after Probio-M9 supplemented. These changes of structure and composition in gut microbiota caused the alternation of microbiome function, of which the sugar degradation, vitamin and amino acid synthesis pathways were significantly enriched. Collectively, this study indicated that Probio-M9 enhanced the host's immunotherapeutic response by promoting the colonization of beneficial bacteria and improving intestinal function.
sample nameSRR15604638_bin.75_metawrap_v1.3_MAG
ENA-CHECKLISTERC000047
ENA-FIRST-PUBLIC2023-01-19
ENA-LAST-UPDATE2023-01-19
External IdSAMEA112229629
INSDC center aliasEMG
INSDC center nameEMG
INSDC first public2023-01-19T12:17:25Z
INSDC last update2023-01-19T12:17:25Z
INSDC statuspublic
Submitter IdSRR15604638_bin.75_metawrap_v1.3_MAG
assembly qualityMany fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly softwarenot provided
binning parametersMaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
binning softwaremetawrap v1.3
broker nameEMG broker account, EMBL-EBI
completeness score95.64
completeness softwareCheckM
contamination score0.73
geographic location (latitude)40.51
geographic location (longitude)110.46
local environmental contextdigestive tube
metagenomic sourcemouse gut metagenome
sample derived fromSAMN20968388
sequencing methodIllumina HiSeq 2500
taxonomic classificationThe taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__
taxonomic identity markermulti-marker approach
Description

This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15604638 of study SRP334008.

BioProject
PRJEB58021
Retrieve all samples from this project

Submission
EBI; 2023-01-20
Accession:
SAMEA112229629
ID:
32807876

BioProject Nucleotide

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